Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphite | - |
Trypanosoma brucei brucei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 pLys S cells | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the monomeric form of TIM does not catalyze any reactions of D-glyceraldehyde 3-phosphate at the enzyme active site, either in the absence or in the presence of phosphite dianion | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C | Trypanosoma brucei brucei | |
0.7 | - |
dihydroxyacetone phosphate | wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C | Trypanosoma brucei brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and CM-Sepharose column chromatography | Trypanosoma brucei brucei |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei brucei | dihydroxyacetone phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Trypanosoma brucei brucei |
Triosephosphate isomerase | - |
Trypanosoma brucei brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
300 | - |
dihydroxyacetone phosphate | wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C | Trypanosoma brucei brucei | |
2100 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C | Trypanosoma brucei brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
430 | - |
dihydroxyacetone phosphate | wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C | Trypanosoma brucei brucei | |
8400 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C | Trypanosoma brucei brucei |