Cloned (Comment) | Organism |
---|---|
expression of the monomeric enzyme in Escherichia coli strain BL21 (DE3) | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
purified recombinant monomeric enzyme by sitting drop method, 0.001 ml of protein solutioncontaining 25 mg/ml protein in 20 mM Tris-HCl , pH 7.4, and 1.0 mM EDTA is mixed with 0.001 ml of reservoir solution containing 100 mM HEPES, pH 7.5, 10% PEG 6000, and 5% MPD, X-ray diffraction structure determination and analysis | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Trypanosoma cruzi | - |
Glycerone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | P52270 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant monomeric enzyme from Escherichia coli strain BL21 (DE3) | Trypanosoma cruzi |
Renatured (Comment) | Organism |
---|---|
unfolding/refolding reactions of monomeric enzyme evaluated using the two-state model | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | active enzyme form | Trypanosoma cruzi |
More | structure determination and analysis of the monomeric enzyme, overview | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
Triosephosphate isomerase | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Trypanosoma cruzi |