Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type rFKBP22, and of mutants rFKBP22D5, rFKBPP22D30, rFKBP22I3, and rFKBP22I6 in Escherichia coli strains SAU1289, SAU1290, SAU1291, SAU1292, and SAU1293, respectively | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of FKBP22 four helix alpha3 mutant variants by various in vitro probes, rFKBP22D5 and rFKBP22D30 are deletion mutants, while rFKBP22I3 and rFKBP22I6 are insertion mutants. The molecular dimensions, dimerization efficiencies, secondary structures, tertiary structures, stabilities, and protein folding abilities of all mutant proteins differ from those of wild-type rFKBP22, but the rapamycin binding affinities of the mutant proteins are affected very little | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22960 | - |
2 * 23300, about, recombinant wild-type enzyme, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D5, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D30, sequence calculation, 2 * 23650, about, recombinant mutant rFKBP22I3, sequence calculation, 2 * 24140, about, recombinant mutant rFKBP22I6, sequence calculation | Escherichia coli |
23300 | - |
2 * 23300, about, recombinant wild-type enzyme, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D5, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D30, sequence calculation, 2 * 23650, about, recombinant mutant rFKBP22I3, sequence calculation, 2 * 24140, about, recombinant mutant rFKBP22I6, sequence calculation | Escherichia coli |
23650 | - |
2 * 23300, about, recombinant wild-type enzyme, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D5, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D30, sequence calculation, 2 * 23650, about, recombinant mutant rFKBP22I3, sequence calculation, 2 * 24140, about, recombinant mutant rFKBP22I6, sequence calculation | Escherichia coli |
24140 | - |
2 * 23300, about, recombinant wild-type enzyme, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D5, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D30, sequence calculation, 2 * 23650, about, recombinant mutant rFKBP22I3, sequence calculation, 2 * 24140, about, recombinant mutant rFKBP22I6, sequence calculation | Escherichia coli |
48910 | - |
recombinant mutant rFKBP22D30, gel filtration | Escherichia coli |
60780 | - |
recombinant wild-type enzyme, gel filtration | Escherichia coli |
65710 | - |
recombinant mutant rFKBP22D5, gel filtration | Escherichia coli |
71180 | - |
recombinant mutant rFKBP22I3, gel filtration | Escherichia coli |
80950 | - |
recombinant mutant rFKBP22I6, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type rFKBP22, and mutants rFKBP22D5, rFKBPP22D30, rFKBP22I3, and rFKBP22I6 from Escherichia coli strain BL21(DE3) derivatives SAU1289, SAU1290, SAU1291, SAU1292, and SAU1293, respectively, by nickel affinity chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes, urea-induced unfolding of wild-type and mutant enzymes follows a two-state mechanism and is reversible in nature, mechanism, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | peptidyl-prolyl cis-trans isomerase FKBP22 binds FK506 and rapamycin, that are both immunosuppressive drugs | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23300, about, recombinant wild-type enzyme, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D5, sequence calculation, 2 * 22960, about, recombinant mutant rFKBP22D30, sequence calculation, 2 * 23650, about, recombinant mutant rFKBP22I3, sequence calculation, 2 * 24140, about, recombinant mutant rFKBP22I6, sequence calculation | Escherichia coli |
More | homology modeling of the three-dimensional enzyme structure, structures of the helix alpha3 mutants, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
FKBP22 | - |
Escherichia coli |
mip-like peptidyl-prolyl cis-trans isomerase | - |
Escherichia coli |
Peptidyl-prolyl cis-trans isomerase | - |
Escherichia coli |
PPIase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | length of helix alpha3 contributes significantly to the preservation of the structure, function, and stability of Escherichia coli FKBP22. Homology modeling of the three-dimensional enzyme structure, overview | Escherichia coli |