Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Corynebacterium glutamicum |
Crystallization (Comment) | Organism |
---|---|
of the wild type and one mutated enzyme (H52A), enzyme contains two domains | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
C61A | site directed mutagenesis | Corynebacterium glutamicum |
D151A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
E144A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
E85A | site directed mutagenesis | Corynebacterium glutamicum |
H148A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
H48A | site directed mutagenesis | Corynebacterium glutamicum |
H52A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
N56A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
R141A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
R222A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
R82A | site directed mutagenesis, partially inactive | Corynebacterium glutamicum |
S78A | site directed mutagenesis, almost completely inactive | Corynebacterium glutamicum |
W44A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
Y76A | site directed mutagenesis, completely inactive | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | probably required for activity | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE, gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NLC1 | wild type | - |
Purification (Comment) | Organism |
---|---|
of the recombinant wild type and mutant enzymes | Corynebacterium glutamicum |
Storage Stability | Organism |
---|---|
at -80°C | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-maleylpyruvate + | - |
Corynebacterium glutamicum | 3-fumarylpyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-maleylpyruvate cis-trans-isomerase | - |
Corynebacterium glutamicum |
maleylpyruvate isomerase | - |
Corynebacterium glutamicum |
MDMPI | mycothiol dependent maleylpyruvate isomerase | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | MSH, mycothiol, 1D-myo-inosityl-1-(N-acetyl-L-cysteinyl)amido-2-deoxy-a-D-glucopyranoside | Corynebacterium glutamicum |