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Literature summary for 5.2.1.14 extracted from
- The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-beta-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency (2018), Plant Sci., 277, 33-42 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| silver acetate | - |
Arabidopsis thaliana |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme is an iron containing protein | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-beta-carotene | Arabidopsis thaliana | - |
9-cis-beta-carotene | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Columbia | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | highest expression in immature flowers | Arabidopsis thaliana | - |
| petal | - |
Arabidopsis thaliana | - |
| plant pedicel | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
| stigma | - |
Arabidopsis thaliana | - |
| style | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-alpha-carotene | - |
Arabidopsis thaliana | 9'-cis-alpha-carotene | - |
? | |
| all-trans-beta,beta-cryptoxanthin | low activity | Arabidopsis thaliana | 9-cis-beta,beta-cryptoxanthin | - |
? | |
| all-trans-beta-carotene | - |
Arabidopsis thaliana | 9-cis-beta-carotene | - |
r | |
| additional information | the enzyme does not isomerize 13-cis-beta-carotene, 15-cis-beta-carotene, all-trans-violaxanthin, all-trans-neoxanthin, all-trans-lutein, 9-cis-beta-apo-10'-carotenal, 9-cis-3-hydroxy-beta-apo-10'-carotenal, 9-cis-alpha-apo-10'-carotenal, and 9-cis-3-hydroxy-alpha-apo-10'-carotenal | Arabidopsis thaliana | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| all-trans-/9-cis-beta-carotene isomerase | - |
Arabidopsis thaliana |
| D27 | - |
Arabidopsis thaliana |
| DWARF27 | - |
Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | in lateral roots, enzyme expression is induced by treatment with auxin and abscisic acid and upon phosphate starvation | up |
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