Literature summary for 5.1.99.7 extracted from

Gabrielsen, M.; Beckham, K.S.; Cogdell, R.J.; Byron, O.; Roe, A.J.
FolX from Pseudomonas aeruginosa is octameric in both crystal and solution (2012), FEBS Lett., 586, 1160-1165.

Search for more literature for 5.1.99.7

Application

Application	Comment	Organism
drug development	the tetrahydrofolate biosynthetic pathway is an established target for important drugs	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
gene folX, recombinant expression	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, vapour diffusion method, mixing of 500 nl of 6 mg/ml protein in 20 mM Tris pH 7.5, 50 mM NaCl, with 500 nl of reservoir solution containing 40% v/v 1,2-propanediol, 100 mM HEPES, pH 7.5, 1 week, at room temperature, X-ray diffraction structure determination and analysis at 3.0 A resolution	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
141500	-	analytical ultracentrifugation and sedimentation equilibrium data	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
7,8-dihydroneopterin 3'-triphosphate	Pseudomonas aeruginosa	-	7,8-dihydromonapterin 3'-triphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HYG7	gene folX	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7,8-dihydroneopterin 3'-triphosphate	-	Pseudomonas aeruginosa	7,8-dihydromonapterin 3'-triphosphate	-	r

Subunits

Subunits	Comment	Organism
octamer	the enzyme protein is an octamer both in the crystal structure, and in solution formed by two tetramers. The monomeric enzyme structure comprises a four-stranded antiparallel sheet, composed of beta1 (residues 10-12 and 16-20), beta2 (residues 33-42), beta3 (residues 98-106) and beta4 (residues 114-121), structure comparisons, overview	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
folX	-	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
metabolism	the epimerase catalyzes one step of the tetrahydrofolate biosynthetic pathway, dihydroneopterin triphosphate is converted to dihydromonapterin triphosphate	Pseudomonas aeruginosa
additional information	the active site of FolX is predicted to comprise residues from two adjacent subunits, which suggests that the tetramer is essential for the activity of the enzyme. Formation of the octamer may play a role in the stability of enzyme FolX	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)