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Literature summary for 5.1.3.8 extracted from

  • Lee, Y.C.; Chien, H.C.; Hsu, W.H.
    Production of N-acetyl-D-neuraminic acid by recombinant whole cells expressing Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase and Escherichia coli N-acetyl-D-neuraminic acid lyase (2007), J. Biotechnol., 129, 453-460.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP 20 fold increase in activity Anabaena sp. CH1
AMPPNP 20 fold increase in activity Anabaena sp. CH1
ATP 0.180 mM for maximal activity Sus scrofa
ATP 20 fold increase in activity, 0.020 mM for maximal activity Anabaena sp. CH1
dATP 20 fold increase in activity Anabaena sp. CH1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anabaena sp. CH1

Inhibitors

Inhibitors Comment Organism Structure
pyruvate 50% reduced activity at 0.2 M pyruvate Anabaena sp. CH1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25.4
-
N-acetyl-D-mannosamine
-
Anabaena sp. CH1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Sus scrofa
Mg2+ no effect Anabaena sp. CH1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
calculated, SDS-PAGE Anabaena sp. CH1

Organism

Organism UniProt Comment Textmining
Anabaena sp. CH1 A4UA16
-
-
Sus scrofa P17560
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by immobilized nickel-ion chromatography Anabaena sp. CH1

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
in the presence of 1 mM ATP Sus scrofa
124
-
in the presence of 1 mM ATP Anabaena sp. CH1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine
-
Sus scrofa N-acetyl-D-mannosamine
-
r
N-acetyl-D-glucosamine
-
Anabaena sp. CH1 N-acetyl-D-mannosamine
-
r

Synonyms

Synonyms Comment Organism
AGE
-
Sus scrofa
AGE
-
Anabaena sp. CH1
bGlcNAc 2-epimerase recombinant protein Sus scrofa
bGlcNAc 2-epimerase recombinant protein Anabaena sp. CH1
GlcNAc 2-epimerase
-
Sus scrofa
GlcNAc 2-epimerase
-
Anabaena sp. CH1
N-acyl-D-glucosamine 2-epimerase
-
Sus scrofa
N-acyl-D-glucosamine 2-epimerase
-
Anabaena sp. CH1
N-acylglucosamine 2-epimerase
-
Sus scrofa
N-acylglucosamine 2-epimerase
-
Anabaena sp. CH1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Anabaena sp. CH1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 more than 90% of maximal activity Anabaena sp. CH1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Anabaena sp. CH1

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 more than 90% of maximal activity Anabaena sp. CH1