Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | required, Km: 0.13 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 under the transcriptional control of taq promoter | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.1 mM, more than 95% inhibition | Homo sapiens | |
Monoiodoacetic acid | 10 mM, more than 95% inhibition | Homo sapiens | |
NEM | 1 mM, more than 95% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.8 | - |
N-acetyl-D-mannosamine | 37°C, pH 7.5 | Homo sapiens | |
21.3 | - |
N-acetyl-D-glucosamine | 37°C, pH 7.5 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Homo sapiens |
85000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.8 | - |
37°C, pH 7.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with mannosamine, glucosamine, mannose, glucose and N-acetyl-galactosamine | Homo sapiens | ? | - |
? | |
additional information | the enzyme inhibits porcine renin activity through formation of a complex of porcine renin with the recombinant enzyme, the so-called high-molecular-weight renin | Homo sapiens | ? | - |
? | |
N-acetyl-D-mannosamine | - |
Homo sapiens | N-acetyl-D-glucosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Homo sapiens |