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Literature summary for 5.1.3.7 extracted from
- Insights into role of the hydrogen bond networks in substrate recognition by UDP-GalNAc 4-epimerases (2011), Biochem. Biophys. Res. Commun., 412, 232-237.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene galE, expression of N-termminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant mutant H305A enzyme, best by microbatch under oil set up and hanging drop vapor diffusion method, from a solution containing 25% PEG 3350, 0.2 M ammonium sulfate, cryoprotection by 15-20% glycerol, X-ray diffraction structure determination and analysis at 2.10 A resolution | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H305A | site-directed mutagenesis, structure determination and analysis, the mutant shows reduced activity with UDP-N-acetylglucosamine | Escherichia coli |
| S144T/H305A | site-directed mutagenesis, the mutant shows highly reduced activity with UDP-N-acetylglucosamine | Escherichia coli |
| S144T/R304G/H305A | site-directed mutagenesis, the mutant shows highly reduced activity with UDP-N-acetylglucosamine | Escherichia coli |
| S144T/R304G/H305A/S306Y | site-directed mutagenesis, the mutant shows no activity with either UDP-GlcNAc or UDP-Glc | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine | Escherichia coli | - |
UDP-N-acetyl-alpha-D-galactosamine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene wbgU | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-termminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine | - |
Escherichia coli | UDP-N-acetyl-alpha-D-galactosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-GalNAc 4-epimerase | - |
Escherichia coli |
| WbgU | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | UDP-hexose 4-epimerases belong to the superfamily of short-chain dehydrogenase/reductase group 3 | Escherichia coli |
| additional information | modeling of specific substrate recognition by UDP-GalNAc 4-epimerases based on mutational analysis and structure, overview | Escherichia coli |
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