Protein Variants | Comment | Organism |
---|---|---|
H95N | the ratio of turnover number to Km-value is 24.1fold lower than that of the wild-type enzyme when activated with ZnCl2 | Escherichia coli |
H97N | the ratio of turnover number to Km-value is 4011fold lower than that of the wild-type enzyme when activated with ZnCl2 | Escherichia coli |
Y229F | the ratio of turnover number to Km-value is 182.5fold lower than that of the wild-type enzyme when activated with ZnCl2 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0471 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM ZnCl2 | Escherichia coli | |
0.091 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme H95N, activated by 0.1 mM ZnCl2 | Escherichia coli | |
0.11 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM CoCl2 | Escherichia coli | |
0.129 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme Y229F, activated by 0.1 mM ZnCl2 | Escherichia coli | |
0.132 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme H97N, activated by 0.1 mM ZnCl2 | Escherichia coli | |
0.415 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MnCl2 | Escherichia coli | |
0.493 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MgCl2 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Zn2+ in mutant enzyme Y229F and in wild-type enzyme, Km-value for wild-type enzyme is 0.00029 mM | Escherichia coli | |
Mg2+ | poorly bound, weak activator, KM-value for wild-type enzyme is 1.35 mM | Escherichia coli | |
Mn2+ | can replace Zn2+ in mutant enzyme Y229F and in wild-type enzyme. Km-value for wild-type enzyme is 0.00054 mM | Escherichia coli | |
additional information | His95 and His97 are likely metal ion ligands | Escherichia coli | |
Zn2+ | physiological activator, KM-value for wild-type enzyme is 0.00017 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ribulose 5-phosphate | - |
Escherichia coli | D-Xylulose 5-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0118 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme Y229F, activated by 0.1 mM ZnCl2 | Escherichia coli | |
0.183 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme H95N, activated by 0.1 mM ZnCl2 | Escherichia coli | |
0.502 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MgCl2 | Escherichia coli | |
2.01 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme H97N, activated by 0.1 mM ZnCl2 | Escherichia coli | |
2.94 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, mutant enzyme H97N, activated by 0.1 mM ZnCl2 | Escherichia coli | |
6.08 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MgCl2 | Escherichia coli | |
17.3 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM ZnCl2 | Escherichia coli | |
21.8 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM CoCl2 | Escherichia coli | |
36.5 | - |
L-ribulose 5-phosphate | 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MnCl2 | Escherichia coli |