BRENDA - Enzyme Database
show all sequences of 5.1.3.29

Crystal structures and enzyme mechanisms of a dual fucose mutarotase/ribose pyranase

Lee, K.H.; Ryu, K.S.; Kim, M.S.; Suh, H.Y.; Ku, B.; Song, Y.L.; Ko, S.; Lee, W., Oh, B.H.; J. Mol. Biol. 391, 178-191 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Escherichia coli
expression in Escherichia coli
Mus musculus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
crystal structures of in complex with L-fucose
Escherichia coli
crystal structures of in complex with L-fucose
Mus musculus
Engineering
Protein Variants
Commentary
Organism
D64N
binding affinity of mutant enzyme is similar to that of wild-type enzyme
Escherichia coli
H22A
binding affinity of mutant enzyme is similar to that of wild-type enzyme
Escherichia coli
Y111F
reduced binding affinity
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P0AEN8
-
-
Mus musculus
Q8R2K1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Mus musculus
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme is a dual fucose mutarotase and ribose pyranase
725706
Escherichia coli
?
-
-
-
-
additional information
the enzyme is a dual fucose mutarotase and ribose pyranase
725706
Mus musculus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
decamer
crystallograpic data, FucU forms a decameric toroid with each active site formed by two adjacent subunits
Escherichia coli
Synonyms
Synonyms
Commentary
Organism
eFucU
-
Escherichia coli
mFucU
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Escherichia coli
expression in Escherichia coli
Mus musculus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structures of in complex with L-fucose
Escherichia coli
crystal structures of in complex with L-fucose
Mus musculus
Engineering (protein specific)
Protein Variants
Commentary
Organism
D64N
binding affinity of mutant enzyme is similar to that of wild-type enzyme
Escherichia coli
H22A
binding affinity of mutant enzyme is similar to that of wild-type enzyme
Escherichia coli
Y111F
reduced binding affinity
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mus musculus
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme is a dual fucose mutarotase and ribose pyranase
725706
Escherichia coli
?
-
-
-
-
additional information
the enzyme is a dual fucose mutarotase and ribose pyranase
725706
Mus musculus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
crystallograpic data, FucU forms a decameric toroid with each active site formed by two adjacent subunits
Escherichia coli
Other publictions for EC 5.1.3.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723846
Higgins
Structure of the fucose mutaro ...
Streptococcus pneumoniae
Acta Crystallogr. Sect. F
67
1524-1530
2011
-
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1
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4
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1
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1
1
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1
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1
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724666
Park
Male-like sexual behavior of f ...
Mus musculus
BMC Genet.
11
62
2010
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1
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1
1
-
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-
725706
Lee
Crystal structures and enzyme ...
Escherichia coli, Mus musculus
J. Mol. Biol.
391
178-191
2009
-
-
2
2
3
-
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-
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2
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2
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2
1
2
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2
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2
3
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2
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2
1
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-
725106
Park
Characterization and role of f ...
Homo sapiens, Mus musculus
Glycobiology
17
955-962
2007
-
-
2
-
-
-
-
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2
-
2
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2
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6
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3
1
2
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2
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2
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2
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6
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3
1
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2
2
-
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725359
Ryu
NMR application probes a novel ...
Escherichia coli
J. Biol. Chem.
279
25544-25548
2004
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1
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1
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1
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1
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3
1
1
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1
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1
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1
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3
1
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2
2