Activating Compound | Comment | Organism | Structure |
---|---|---|---|
UDP-GlcNAc | allosteric activitation by substrate via direct interactions between UDP and substrate | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
ternary complex between the UDP-GlcNAc 2-epimerase, its substrate UDP-GlcNAc and the reaction intermediate UDP, at 1.7 A resolution. Direct interactions between the substrate and UDP via two hydrogen bonds to the alpha- and beta-phosphates of the adjacent UDP molecule, and between the complex and highly conserved enzyme residues. The binding of UDP-GlcNAc is associated with conformational changes in the active site of the enzyme | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
H242A | dramatic decrease in catalytic efficiency | Bacillus anthracis |
H44Q | dramatic decrease in catalytic efficiency | Bacillus anthracis |
Q43A | dramatic decrease in catalytic efficiency | Bacillus anthracis |
Q70A | dramatic decrease in catalytic efficiency | Bacillus anthracis |
R210A | dramatic decrease in catalytic efficiency | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
UDP-N-acetyl-D-glucosamine | wild-type | Bacillus anthracis | |
7.3 | - |
UDP-N-acetyl-D-glucosamine | mutant H44Q | Bacillus anthracis | |
10.4 | - |
UDP-N-acetyl-D-glucosamine | mutant H242A | Bacillus anthracis | |
11.1 | - |
UDP-N-acetyl-D-glucosamine | mutant Q70A | Bacillus anthracis | |
17.2 | - |
UDP-N-acetyl-D-glucosamine | mutant Q43A | Bacillus anthracis | |
20.9 | - |
UDP-N-acetyl-D-glucosamine | mutant R210A | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine | non-hydrolizing | Bacillus anthracis | UDP-N-acetyl-D-mannosamine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
UDP-N-acetyl-D-glucosamine | mutant R210A | Bacillus anthracis | |
0.022 | - |
UDP-N-acetyl-D-glucosamine | mutant H242A | Bacillus anthracis | |
1.2 | - |
UDP-N-acetyl-D-glucosamine | mutant Q43A | Bacillus anthracis | |
2.06 | - |
UDP-N-acetyl-D-glucosamine | mutant Q70A | Bacillus anthracis | |
4 | - |
UDP-N-acetyl-D-glucosamine | mutant H44Q | Bacillus anthracis | |
7.9 | - |
UDP-N-acetyl-D-glucosamine | wild-type | Bacillus anthracis |