Literature summary for 5.1.1.7 extracted from

Liechti, G.; Singh, R.; Rossi, P.; Gray, M.; Adams, N.; Maurelli, A.
Chlamydia trachomatis dapF encodes a bifunctional enzyme capable of both D-glutamate racemase and diaminopimelate epimerase activities (2018), mBio, 9, e00204-18 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene dapF, sequence comparisons and phylogenetic analysis, Chlamydia trachomatis dapF restores growth in an Escherichia coli D-glutamate auxotroph strain WM335 also lacking endogenaous gene dapF, expression under the control of an arabinose-inducible promoter, the recombinant enzyme is capable of both DAP epimerase and D-glut racemase activities, DapFCT is also capable of racemizing D-Glu to L-Glu	Chlamydia trachomatis

Cloned (Comment)

Organism

gene dapF, sequence comparisons and phylogenetic analysis, Chlamydia trachomatis dapF restores growth in an Escherichia coli D-glutamate auxotroph strain WM335 also lacking endogenaous gene dapF, expression under the control of an arabinose-inducible promoter, the recombinant enzyme is capable of both DAP epimerase and D-glut racemase activities, DapFCT is also capable of racemizing D-Glu to L-Glu

Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
LL-2,6-Diaminoheptanedioate	Chlamydia trachomatis	-	meso-Diaminoheptanedioate	-	r
additional information	Chlamydia trachomatis	Chlamydia trachomatis dapF encodes a bifunctional enzyme capable of both D-glutamate racemase, EC 5.1.1.3, and diaminopimelate epimerase activities. DAP and glutamate appear to be competitive substrates, indicating that they share an active site despite the racemase reaction requiring the pyridoxal 5'-phosphate cofactor	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlamydia trachomatis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
LL-2,6-Diaminoheptanedioate	-	Chlamydia trachomatis	meso-Diaminoheptanedioate	-	r
additional information	Chlamydia trachomatis dapF encodes a bifunctional enzyme capable of both D-glutamate racemase, EC 5.1.1.3, and diaminopimelate epimerase activities. DAP and glutamate appear to be competitive substrates, indicating that they share an active site despite the racemase reaction requiring the pyridoxal 5'-phosphate cofactor	Chlamydia trachomatis	?	-	?

Synonyms

Synonyms	Comment	Organism
DapF	-	Chlamydia trachomatis
DapFCT	-	Chlamydia trachomatis

General Information

General Information	Comment	Organism
metabolism	diaminopimelate epimerase (DapF) catalyzes the final step in the synthesis of meso-diaminopimelate, an amino acid unique to peptidoglycan, and synthesizes D-glutamate, EC 5.1.1.3	Chlamydia trachomatis
additional information	the structure of Chlamydia DAP epimerase exhibits significant remodeling in the substrate-binding pocket, overview	Chlamydia trachomatis