Cloned (Comment) | Organism |
---|---|
gene OEOE0162, DNA and amino acid sequence determination and analysis, expression of the enzyme in Escherichia coli strain BL21 | Oenococcus oeni |
Protein Variants | Comment | Organism |
---|---|---|
T224I | site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme | Oenococcus oeni |
T224I/W355Y | site-directed mutagenesis, the double mutant shows significantly decreased activity compared to the wild-type enzyme | Oenococcus oeni |
W355Y | site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme | Oenococcus oeni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 2 | D-Lysine | pH 8.0, 30°C, recombinant mutant T224I | Oenococcus oeni | |
2 | 3 | L-lysine | pH 8.0, 30°C, recombinant mutant W355Y | Oenococcus oeni | |
9.8 | - |
D-Lysine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
11 | - |
L-lysine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
15 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant T224I | Oenococcus oeni | |
21 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant W355Y | Oenococcus oeni | |
22 | - |
D-ornithine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
27 | - |
L-ornithine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
27 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant T224I/W355Y | Oenococcus oeni | |
31 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant T224I/W355Y | Oenococcus oeni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Oenococcus oeni | - |
D-lysine | - |
? | |
additional information | Oenococcus oeni | the Oenococcus oeni lysine racemase has a specific activity towards basic amino acids, residues Thr224 and Trp355 are important for enzyme substrate specificity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oenococcus oeni | Q04HB7 | gene OEOE0162 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lysine | - |
Oenococcus oeni | L-lysine | - |
r | |
D-ornithine | - |
Oenococcus oeni | L-ornithine | - |
r | |
L-lysine | - |
Oenococcus oeni | D-lysine | - |
? | |
L-lysine | - |
Oenococcus oeni | D-lysine | - |
r | |
L-Ornithine | - |
Oenococcus oeni | D-Ornithine | - |
r | |
additional information | the Oenococcus oeni lysine racemase has a specific activity towards basic amino acids, residues Thr224 and Trp355 are important for enzyme substrate specificity | Oenococcus oeni | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oenococcus oeni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant T224I/W355Y | Oenococcus oeni | |
0.16 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant T224I/W355Y | Oenococcus oeni | |
0.28 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant T224I | Oenococcus oeni | |
0.32 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant T224I | Oenococcus oeni | |
0.88 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant W355Y | Oenococcus oeni | |
1.03 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant W355Y | Oenococcus oeni | |
1.83 | - |
L-ornithine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
1.83 | - |
D-ornithine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
2.83 | - |
D-Lysine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
3.5 | - |
L-lysine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Oenococcus oeni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Oenococcus oeni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant T224I/W355Y | Oenococcus oeni | |
0.006 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant T224I/W355Y | Oenococcus oeni | |
0.023 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant T224I | Oenococcus oeni | |
0.025 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant T224I | Oenococcus oeni | |
0.042 | - |
D-Lysine | pH 8.0, 30°C, recombinant mutant W355Y | Oenococcus oeni | |
0.045 | - |
L-lysine | pH 8.0, 30°C, recombinant mutant W355Y | Oenococcus oeni | |
0.07 | - |
L-ornithine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
0.08 | - |
D-ornithine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
0.28 | - |
D-Lysine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni | |
0.32 | - |
L-lysine | pH 8.0, 30°C, recombinant wild-type enzyme | Oenococcus oeni |