BRENDA - Enzyme Database
show all sequences of 4.99.1.5

A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4

Xie, S.X.; Kato, Y.; Komeda, H.; Yoshida, S.; Asano, Y.; Biochemistry 42, 12056-12066 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.13fold
Rhodococcus globerulus
cysteamine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 3.93fold
Rhodococcus globerulus
duroquinone
activates
Rhodococcus globerulus
FAD
1 mM, 1.2fold activation in presence of Na2S, 4.5fold in absence of Na2S
Rhodococcus globerulus
FMN
1 mM, 1.7fold activation in presence of Na2S, 2.4fold in absence of Na2S
Rhodococcus globerulus
L-cysteine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.39fold
Rhodococcus globerulus
additional information
not activated by NaHSO3, Na2SO4, NaHSO4 or Na2S2O7
Rhodococcus globerulus
Na2S
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.35fold
Rhodococcus globerulus
Na2S2O4
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.13fold
Rhodococcus globerulus
Na2S2O5
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 5.84fold
Rhodococcus globerulus
Na2SO3
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 7.51fold
Rhodococcus globerulus
reducing reagent
requirement, activates
Rhodococcus globerulus
riboflavin
1 mM, 1.1fold activation in presence of Na2S, 4.2fold in absence of Na2S
Rhodococcus globerulus
thioglycerol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 4.06fold
Rhodococcus globerulus
vitamin K3
activates
Rhodococcus globerulus
Application
Application
Commentary
Organism
synthesis
may be a useful biocatalyst for the production of various nitriles from the corresponding aldoximes
Rhodococcus globerulus
Cloned(Commentary)
Cloned (Commentary)
Organism
oxd gene, overexpression in Escherichia coli JM109, sequencing, gene cluster organisation
Rhodococcus globerulus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2,3-benzenetriol
slight inhibition
Rhodococcus globerulus
Cd2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
Co2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
Cu+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
Cu2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
dimethylphenylenediamine
slight inhibition
Rhodococcus globerulus
guaiacol
slight inhibition
Rhodococcus globerulus
heavy metal ion
1 mM
Rhodococcus globerulus
miconazole
slight inhibition
Rhodococcus globerulus
additional information
not inhibited by hydrazine, KCN, NaN3, and NH2OH
Rhodococcus globerulus
nitroblue tetrazolium
slight inhibition
Rhodococcus globerulus
p-phenylenediamine
slight inhibition
Rhodococcus globerulus
phenazine methosulfate
slight inhibition
Rhodococcus globerulus
phenylhydrazine
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
sulfhydryl reagent
1 mM
Rhodococcus globerulus
tetramethylphenylenediamine
slight inhibition
Rhodococcus globerulus
Tiron
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
trimethylhydroquinone
slight inhibition
Rhodococcus globerulus
Zn2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Km for the activating Na2S is 0.0408 mM
Rhodococcus globerulus
1.13
-
(E/Z)-n-valeraldoxime
pH 7, 30°C
Rhodococcus globerulus
1.13
-
(E/Z)-cyclohexanecarboxaldehyde oxime
pH 7, 30°C
Rhodococcus globerulus
1.4
-
(Z)-phenylacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
1.73
-
(E/Z)-n-butyraldoxime
pH 7, 30°C
Rhodococcus globerulus
2.31
-
(Z)-3-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
2.94
-
(E/Z)-n-capronaldoxime
pH 7, 30°C
Rhodococcus globerulus
3.23
-
(E/Z)-mandelaldoxime
pH 7, 30°C
Rhodococcus globerulus
3.91
-
(E/Z)-indoleacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
3.97
-
(E/Z)-isovaleraldoxime
pH 7, 30°C
Rhodococcus globerulus
5.13
-
(E/Z)-propionaldoxime
pH 7, 30°C
Rhodococcus globerulus
5.54
-
(E/Z)-isobutyraldoxime
pH 7, 30°C
Rhodococcus globerulus
6.76
-
(E/Z)-isocapronaldoxime
pH 7, 30°C
Rhodococcus globerulus
11.9
-
(E/Z)-2-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
20
-
(E)-pyridine-3-aldoxime
pH 7, 30°C
Rhodococcus globerulus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Co2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Cu+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Cu2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Fe2+
heme iron is present in a reduced form, 1 mM, activates
Rhodococcus globerulus
Fe3+
1 mM, activates
Rhodococcus globerulus
Zn2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39892
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
42000
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
76200
-
wild-type OxdRG, gel filtration
Rhodococcus globerulus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
alkylaldoxime
Rhodococcus globerulus
responsible for the metabolism of alkylaldoxime
alkylnitrile + H2O
-
Rhodococcus globerulus
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus globerulus
Q76EV4
-
-
Rhodococcus globerulus A-4
Q76EV4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
wild-type OxdRG: 104fold, recombinant OxdRG expressed in Escherichia coli JM109: 14.9fold
Rhodococcus globerulus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0879
-
pH 7, 30°C, wild-type OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
2.18
-
pH 7, 30°C, recombinant OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(E)-pyridine-3-aldoxime
not Z-form, poor substrate, 0.78% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
pyridine-3-nitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-2-phenylpropionaldoxime
5.23% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
2-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus
?
-
650189
Rhodococcus globerulus
?
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus A-4
?
-
650189
Rhodococcus globerulus A-4
?
(E/Z)-indoleacetaldoxime
7.29% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
indoleacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-isobutyraldoxime
26.5% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isobutyronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-isocapronaldoxime
88.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isocaprononitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-isovaleraldoxime
58.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isovaleronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-mandelaldoxime
3.09% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
mandelonitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
(E/Z)-n-capronaldoxime
64.2% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-caprononitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
(E/Z)-propionaldoxime
7.76% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
propiononitrile + H2O
-
650189
Rhodococcus globerulus
?
(Z)-3-phenylpropionaldoxime
67.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
3-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus
nitrile + H2O
-
650189
Rhodococcus globerulus
?
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus A-4
nitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
alkylaldoxime
responsible for the metabolism of alkylaldoxime
650189
Rhodococcus globerulus
alkylnitrile + H2O
-
650189
Rhodococcus globerulus
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
Synonyms
Synonyms
Commentary
Organism
alkylaldoxime dehydratase
-
Rhodococcus globerulus
OxdRG
-
Rhodococcus globerulus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
around, 0.1 M potassium phosphate buffer, pH 8
Rhodococcus globerulus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
30 min, 0.1 M potassium phosphate buffer, pH 8, 35% loss of activity
Rhodococcus globerulus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
0.1 M potassium phosphate buffer
Rhodococcus globerulus
Cofactor
Cofactor
Commentary
Organism
Structure
heme b
prosthetic group, protoheme IX, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
protoheme IX
prosthetic group, heme b, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.13fold
Rhodococcus globerulus
cysteamine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 3.93fold
Rhodococcus globerulus
duroquinone
activates
Rhodococcus globerulus
FAD
1 mM, 1.2fold activation in presence of Na2S, 4.5fold in absence of Na2S
Rhodococcus globerulus
FMN
1 mM, 1.7fold activation in presence of Na2S, 2.4fold in absence of Na2S
Rhodococcus globerulus
L-cysteine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.39fold
Rhodococcus globerulus
additional information
not activated by NaHSO3, Na2SO4, NaHSO4 or Na2S2O7
Rhodococcus globerulus
Na2S
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.35fold
Rhodococcus globerulus
Na2S2O4
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.13fold
Rhodococcus globerulus
Na2S2O5
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 5.84fold
Rhodococcus globerulus
Na2SO3
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 7.51fold
Rhodococcus globerulus
reducing reagent
requirement, activates
Rhodococcus globerulus
riboflavin
1 mM, 1.1fold activation in presence of Na2S, 4.2fold in absence of Na2S
Rhodococcus globerulus
thioglycerol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 4.06fold
Rhodococcus globerulus
vitamin K3
activates
Rhodococcus globerulus
Application (protein specific)
Application
Commentary
Organism
synthesis
may be a useful biocatalyst for the production of various nitriles from the corresponding aldoximes
Rhodococcus globerulus
Cloned(Commentary) (protein specific)
Commentary
Organism
oxd gene, overexpression in Escherichia coli JM109, sequencing, gene cluster organisation
Rhodococcus globerulus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme b
prosthetic group, protoheme IX, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
protoheme IX
prosthetic group, heme b, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2,3-benzenetriol
slight inhibition
Rhodococcus globerulus
Cd2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
Co2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
Cu+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
Cu2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
dimethylphenylenediamine
slight inhibition
Rhodococcus globerulus
guaiacol
slight inhibition
Rhodococcus globerulus
heavy metal ion
1 mM
Rhodococcus globerulus
miconazole
slight inhibition
Rhodococcus globerulus
additional information
not inhibited by hydrazine, KCN, NaN3, and NH2OH
Rhodococcus globerulus
nitroblue tetrazolium
slight inhibition
Rhodococcus globerulus
p-phenylenediamine
slight inhibition
Rhodococcus globerulus
phenazine methosulfate
slight inhibition
Rhodococcus globerulus
phenylhydrazine
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
sulfhydryl reagent
1 mM
Rhodococcus globerulus
tetramethylphenylenediamine
slight inhibition
Rhodococcus globerulus
Tiron
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
trimethylhydroquinone
slight inhibition
Rhodococcus globerulus
Zn2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Km for the activating Na2S is 0.0408 mM
Rhodococcus globerulus
1.13
-
(E/Z)-n-valeraldoxime
pH 7, 30°C
Rhodococcus globerulus
1.13
-
(E/Z)-cyclohexanecarboxaldehyde oxime
pH 7, 30°C
Rhodococcus globerulus
1.4
-
(Z)-phenylacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
1.73
-
(E/Z)-n-butyraldoxime
pH 7, 30°C
Rhodococcus globerulus
2.31
-
(Z)-3-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
2.94
-
(E/Z)-n-capronaldoxime
pH 7, 30°C
Rhodococcus globerulus
3.23
-
(E/Z)-mandelaldoxime
pH 7, 30°C
Rhodococcus globerulus
3.91
-
(E/Z)-indoleacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
3.97
-
(E/Z)-isovaleraldoxime
pH 7, 30°C
Rhodococcus globerulus
5.13
-
(E/Z)-propionaldoxime
pH 7, 30°C
Rhodococcus globerulus
5.54
-
(E/Z)-isobutyraldoxime
pH 7, 30°C
Rhodococcus globerulus
6.76
-
(E/Z)-isocapronaldoxime
pH 7, 30°C
Rhodococcus globerulus
11.9
-
(E/Z)-2-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
20
-
(E)-pyridine-3-aldoxime
pH 7, 30°C
Rhodococcus globerulus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Co2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Cu+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Cu2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Fe2+
heme iron is present in a reduced form, 1 mM, activates
Rhodococcus globerulus
Fe3+
1 mM, activates
Rhodococcus globerulus
Zn2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39892
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
42000
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
76200
-
wild-type OxdRG, gel filtration
Rhodococcus globerulus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
alkylaldoxime
Rhodococcus globerulus
responsible for the metabolism of alkylaldoxime
alkylnitrile + H2O
-
Rhodococcus globerulus
?
Purification (Commentary) (protein specific)
Commentary
Organism
wild-type OxdRG: 104fold, recombinant OxdRG expressed in Escherichia coli JM109: 14.9fold
Rhodococcus globerulus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0879
-
pH 7, 30°C, wild-type OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
2.18
-
pH 7, 30°C, recombinant OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(E)-pyridine-3-aldoxime
not Z-form, poor substrate, 0.78% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
pyridine-3-nitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-2-phenylpropionaldoxime
5.23% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
2-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus
?
-
650189
Rhodococcus globerulus
?
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus A-4
?
-
650189
Rhodococcus globerulus A-4
?
(E/Z)-indoleacetaldoxime
7.29% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
indoleacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-isobutyraldoxime
26.5% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isobutyronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-isocapronaldoxime
88.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isocaprononitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-isovaleraldoxime
58.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isovaleronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-mandelaldoxime
3.09% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
mandelonitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
(E/Z)-n-capronaldoxime
64.2% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-caprononitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus
?
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
(E/Z)-propionaldoxime
7.76% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
propiononitrile + H2O
-
650189
Rhodococcus globerulus
?
(Z)-3-phenylpropionaldoxime
67.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
3-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus
nitrile + H2O
-
650189
Rhodococcus globerulus
?
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus A-4
nitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
alkylaldoxime
responsible for the metabolism of alkylaldoxime
650189
Rhodococcus globerulus
alkylnitrile + H2O
-
650189
Rhodococcus globerulus
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
around, 0.1 M potassium phosphate buffer, pH 8
Rhodococcus globerulus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
30 min, 0.1 M potassium phosphate buffer, pH 8, 35% loss of activity
Rhodococcus globerulus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
0.1 M potassium phosphate buffer
Rhodococcus globerulus
Other publictions for EC 4.99.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747550
Miao
Kemp elimination catalyzed by ...
Bacillus sp. OxB-1, Rhodococcus sp. N-771, Pseudomonas chlororaphis
ChemBioChem
18
451-454
2017
-
3
3
-
6
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
6
-
-
-
3
-
-
-
-
3
3
3
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
749107
Yamada
New function of aldoxime dehy ...
Pseudomonas chlororaphis
PLoS ONE
12
e0175846
2017
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730824
Nomura
Crystal structure of aldoxime ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
Proc. Natl. Acad. Sci. USA
110
2810-2815
2013
-
-
1
1
13
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
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1
-
-
-
-
-
1
1
1
13
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730254
Pan
QM/MM study on the catalytic m ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
J. Phys. Chem. B
116
5689-5693
2012
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
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1
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-
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-
-
1
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730257
Liao
Why is the oxidation state of ...
Rhodococcus sp.
J. Phys. Chem. B
116
9396-9408
2012
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730252
Pinakoulaki
Aldoxime dehydratase: probing ...
Rhodococcus sp.
J. Phys. Chem. B
115
13012-13018
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704573
Sawai
X-ray crystal structure of mic ...
Rhodococcus erythropolis
J. Biol. Chem.
284
32089-32096
2009
-
1
1
1
5
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
1
2
-
-
-
6
-
-
-
1
-
-
-
-
1
1
1
1
5
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
4
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
662580
Kobayashi
Spectroscopic and substrate bi ...
Bacillus sp. (in: Bacteria), Rhodococcus sp., Bacillus sp. (in: Bacteria) OxB-1
J. Inorg. Biochem.
100
1069-1074
2006
-
-
2
-
-
-
-
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
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-
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2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663258
Konishi
Discovery of a reaction interm ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
Proc. Natl. Acad. Sci. USA
103
564-568
2006
-
1
1
-
-
-
-
-
-
-
-
-
-
28
-
-
2
-
-
-
-
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6
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677719
Kato
Molecular and enzymatic analys ...
Pseudomonas sp., Pseudomonas sp. K-9
Appl. Microbiol. Biotechnol.
70
92-101
2006
-
-
1
-
-
-
4
11
-
6
3
-
-
5
-
-
1
-
-
-
-
-
18
1
3
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
11
-
6
3
-
-
-
-
1
-
-
-
-
18
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
661752
Oinuma
Stopped-flow spectrophotometri ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
FEBS Lett.
579
1394-1398
2005
-
-
-
-
-
-
-
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13
-
-
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2
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1
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1
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-
-
1
-
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-
-
-
-
-
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-
-
2
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-
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-
-
-
-
-
-
-
-
-
-
-
-
661737
Oinuma
Heme environment in aldoxime d ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
FEBS Lett.
568
44-48
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
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1
-
-
-
-
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1
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-
-
-
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1
-
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-
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1
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662292
Konishi
Identification of crucial hist ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
J. Biol. Chem.
279
47619-47625
2004
-
-
-
-
7
-
-
-
-
-
-
-
-
13
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648793
Oinuma
Novel aldoxime dehydratase inv ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
J. Biol. Chem.
278
29600-29608
2003
1
-
1
-
-
-
11
3
-
2
4
2
-
26
-
-
1
-
-
-
2
-
13
1
2
1
-
7
3
2
1
-
2
-
-
-
1
-
1
2
-
-
-
-
11
-
3
-
2
4
2
-
-
-
1
-
-
2
-
13
1
1
-
7
3
2
1
-
-
-
-
-
-
-
-
650189
Xie
A gene cluster responsible for ...
Rhodococcus globerulus, Rhodococcus globerulus A-4
Biochemistry
42
12056-12066
2003
15
1
1
-
-
-
19
15
-
7
3
1
-
6
-
-
1
-
-
-
2
-
21
1
2
1
-
1
-
1
-
-
2
-
-
-
15
1
1
2
-
-
-
-
19
-
15
-
7
3
1
-
-
-
1
-
-
2
-
21
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-