BRENDA - Enzyme Database
show all sequences of 4.99.1.4

Identification and characterization of the missing terminal enzyme for siroheme biosynthesis in alpha-proteobacteria

Bali, S.; Rollauer, S.; Roversi, P.; Raux-Deery, E.; Lea, S.M.; Warren, M.J.; Ferguson, S.J.; Mol. Microbiol. 92, 153-163 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21 codonplus (RIPL) cells
Paracoccus pantotrophus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
sitting drop vapor diffusion method, using in 0.2 M imidazole malate pH 5.5, 24% (w/v) PEG 600 at a ratio of 50:50 ratio of protein : mother liquor and 10% ethylene glycol
Paracoccus pantotrophus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, SDS-PAGE
Paracoccus pantotrophus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
sirohydrochlorin + Fe2+
Paracoccus pantotrophus
-
siroheme + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Paracoccus pantotrophus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
chelating Sepharose column chromatography
Paracoccus pantotrophus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
sirohydrochlorin + Fe2+
-
730419
Paracoccus pantotrophus
siroheme + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 20000, SDS-PAGE
Paracoccus pantotrophus
Synonyms
Synonyms
Commentary
Organism
CbiX
-
Paracoccus pantotrophus
CysG
-
Paracoccus pantotrophus
Met8p
-
Paracoccus pantotrophus
SirB
-
Paracoccus pantotrophus
sirohydrochlorin-ferrochelatase
-
Paracoccus pantotrophus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 codonplus (RIPL) cells
Paracoccus pantotrophus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, using in 0.2 M imidazole malate pH 5.5, 24% (w/v) PEG 600 at a ratio of 50:50 ratio of protein : mother liquor and 10% ethylene glycol
Paracoccus pantotrophus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, SDS-PAGE
Paracoccus pantotrophus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
sirohydrochlorin + Fe2+
Paracoccus pantotrophus
-
siroheme + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
chelating Sepharose column chromatography
Paracoccus pantotrophus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
sirohydrochlorin + Fe2+
-
730419
Paracoccus pantotrophus
siroheme + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 20000, SDS-PAGE
Paracoccus pantotrophus
Other publictions for EC 4.99.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748869
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351-359
2016
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1
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3
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
730419
Bali
Identification and characteriz ...
Paracoccus pantotrophus
Mol. Microbiol.
92
153-163
2014
-
-
1
1
-
-
-
-
-
-
1
1
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1
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1
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1
1
5
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1
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1
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1
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1
1
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729160
Saha
Characterization of the evolut ...
Arabidopsis thaliana
Biochem. J.
444
227-237
2012
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1
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5
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1
1
2
1
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5
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6
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6
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1
1
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716791
Romao
Evolution in a family of chela ...
Bacillus megaterium
Proc. Natl. Acad. Sci. USA
108
97-102
2011
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1
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696120
Lobo
Functional characterization of ...
no activity in Desulfovibrio vulgaris
Biochem. J.
420
317-325
2009
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1
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665692
Raux-Deery
Identification and characteriz ...
Arabidopsis thaliana
J. Biol. Chem.
280
4713-4721
2005
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1
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2
1
1
2
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1
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4
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1
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3
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649745
Raux
Identification and functional ...
Bacillus megaterium, Bacillus megaterium DSM 509
Biochem. J.
370
505-516
2003
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8
1
1
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1
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1
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1
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2
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8
1
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1
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653297
Stroupe
CysG structure reveals tetrapy ...
Salmonella enterica
Nat. Struct. Biol.
10
1064-1073
2003
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1
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1
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3
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3
1
1
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1
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1
2
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1
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1
1
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3
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3
1
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644283
Schubert
The structure of Saccharomyces ...
Saccharomyces cerevisiae
EMBO J.
21
2068-2075
2002
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1
1
3
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4
1
2
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1
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1
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1
3
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1
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4
1
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1
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649817
Schubert
Structural diversity in metal ...
Saccharomyces cerevisiae
Biochem. Soc. Trans.
30
595-600
2002
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1
2
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1
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2
1
2
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1
2
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