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Literature summary for 4.6.1.2 extracted from
- A glutamate-substituted mutant mimics the phosphorylated and active form of guanylyl cyclase-A (2017), Mol. Pharmacol., 92, 67-74 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transfection of HEK-293T cell | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S473A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S473E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S473E/S497E/T500E/S502E/S506E/S510E/T513E | 2fold increase of Km value compared to wild-type | Homo sapiens |
| S487A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S487E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S497A | mutation of potential phosphorylation site, mutation increases Km value about 4fold | Homo sapiens |
| S497E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S497E/T500E/S502E/S506E/S510E/T513E | mutations in potential phosphorylation sites, about 20% of the activity of phosphorylated wild-type | Homo sapiens |
| S502A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S502E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S506A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S506E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S510A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| S510E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| T500A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| T500E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| T513A | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
| T513E | mutation of potential phosphorylation site, reduces vmax value by 13-55% | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.054 | - |
GTP | wild-type, 37°C, temperature not specified in the publication, pH not specified in the publication | Homo sapiens |  |
| 0.07 | - |
GTP | mutant S502A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.088 | - |
GTP | mutant S473A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.099 | - |
GTP | mutant S487A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.107 | - |
GTP | mutant T500E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.111 | - |
GTP | mutant T513E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.113 | - |
GTP | mutant S502E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.116 | - |
GTP | mutant S473E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.144 | - |
GTP | mutant S487E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.15 | - |
GTP | mutant T500A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.162 | - |
GTP | mutant S510E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.165 | - |
GTP | mutant T513A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.184 | - |
GTP | mutant S497E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.195 | - |
GTP | mutant S510A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.196 | - |
GTP | mutant S506E, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.221 | - |
GTP | mutant S506A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
| 0.389 | - |
GTP | mutant S497A, temperature not specified in the publication, pH not specified in the publication | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P16066 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Homo sapiens | 3',5'-cGMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NPR A | - |
Homo sapiens |
| NPR1 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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