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Literature summary for 4.6.1.2 extracted from

  • Baskaran, P.; Heckler, E.J.; van den Akker, F.; Beuve, A.
    Aspartate 102 in the heme domain of soluble guanylyl cyclase has a key role in NO activation (2011), Biochemistry, 50, 4291-4297.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
2-(N,N-diethylamino)diazenolate-2-oxide the wild type enzyme shows 59fold stimulation of activity at 0.001 mM 2-(N,N-diethylamino)diazenolate-2-oxide Rattus norvegicus
5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol the wild type enzyme shows 6fold stimulation of activity at 0.1 mM Rattus norvegicus
protoporphyrin-IX the wild type enzyme shows 1.9fold stimulation of activity at 0.1 mM and 12fold stimulation at 0.01 mM Rattus norvegicus
S-nitroso-N-acetylpenicillamine the wild type enzyme shows 14.5fold stimulation of activity at 0.1 mM Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf21 insect cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D102A the mutant shows 70% of wild type activity Rattus norvegicus
D102E the mutant shows 32% of wild type activity Rattus norvegicus
D102N the mutant shows 20.5% of wild type activity Rattus norvegicus
F120A the mutant shows 73% of wild type activity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt affinity column chromatography and Mono Q anion exchange column chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.047
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
0.073
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
0.087
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
0.108
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
0.128
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.152
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.16
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
0.161
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
0.168
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
0.169
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
0.172
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.202
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.229
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.229
-
 wild type enzyme, at pH 8.5 and 33°C, in the absence of any stimulator Rattus norvegicus
0.231
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.278
-
 mutant enzyme F120A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.292
-
 mutant enzyme D102E, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.354
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
0.431
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM protoporphyrin-IX Rattus norvegicus
0.662
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
1.063
-
 mutant enzyme D102N, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
1.232
-
 mutant enzyme D102A, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
1.43
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
3.328
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus
3.402
-
 wild type enzyme, at pH 8.5 and 33°C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Rattus norvegicus 3',5'-cyclic-GMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
sGC
-
 Rattus norvegicus
soluble guanylate cyclase
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Rattus norvegicus