Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,2-diethyl-1-nitroso-oxyhydrazine | 70-80% activation at 0.01 mM in presence of 2 mM GSH | Bos taurus | |
NO | binding of NO to a regulatory heme group at the beta-subunit of the protein results in 100fold stimulation of cGMP formation, NO is involved in the vascular NO/cGMP signalling, dysfunction of the signaling is thought to contribute to a wide variety of cardiovascular disorders | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DNIC-G | a dinitrosyl iron complex, solutions contain 18fold molar excess of free thiosulfate, inhibition of the NO-stimulated enzyme, inhibited by GSH | Bos taurus | |
DNIC-Y | a dinitrosyl iron complex, no free thiosulfate in solutions of the binuclear complex DNIC-Y, inhibition of the NO-stimulated enzyme, inhibited by GSH | Bos taurus | |
additional information | desensitization of the enzyme to NO and inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation through dinitrosyl iron complexes, which is slowly reversible by GSH, overview | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Bos taurus | - |
3',5'-cyclic GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from lung | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Bos taurus | 3',5'-cyclic GMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Bos taurus |
soluble guanylate cyclase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bos taurus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
pH 7.4, 37°C, inhibition of NO-stimulated enzyme | Bos taurus | DNIC-G | |
0.01 | - |
pH 7.4, 37°C, inhibition of NO-stimulated enzyme | Bos taurus | DNIC-Y |