Literature summary for 4.6.1.2 extracted from

Boon, E.M.; Davis, J.H.; Tran, R.; Karow, D.S.; Huang, S.H.; Pan, D.; Miazgowicz, M.M.; Mathies, R.A.; Marletta, M.A.
Nitric oxide binding to prokaryotic homologs of the soluble guanylate cyclase beta1 H-NOX domain (2006), J. Biol. Chem., 281, 21892-21902.

Search for more literature for 4.6.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
NO	is bound in the distal heme pocket of the L2 H-NOX fold, NO dissociates from the distal side of the heme in both 5- and 6-coordinate complexes	Legionella pneumophila
NO	is bound in the distal heme pocket of the L2 H-NOX fold, NO dissociates from the distal side of the heme in both 5- and 6-coordinate complexes	Nostoc punctiforme

Cloned(Commentary)

Cloned (Comment)	Organism
into pET-20b vector	Legionella pneumophila
into pET-20b vector	Nostoc punctiforme

Protein Variants

Protein Variants	Comment	Organism
F142Y	L2 H-NOX mutant, shift of the equilibrium of the FeII-NO complex at 20°C exclusively to the 6-coordinate complex	Legionella pneumophila

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	forms a temperature-dependent mixture of 5- and 6-coordinate FeII-NO complexes	Nostoc punctiforme
Fe2+	L1 H-NOX forms a temperature-independent 5-coordinate FeII-NO complex, L2 H-NOX forms a temperature-dependent mixture of 5- and 6-coordinate FeII-NO complexes	Legionella pneumophila

Organism

Organism	UniProt	Comment	Textmining
Legionella pneumophila	-	-	-
Nostoc punctiforme	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Nostoc punctiforme
by metal affinity chromatography followed by gel filtration	Legionella pneumophila

Synonyms

Synonyms	Comment	Organism
H-NOX	-	Nostoc punctiforme
L1 H-NOX	-	Legionella pneumophila
L2 H-NOX	-	Legionella pneumophila
sGC	-	Legionella pneumophila
sGC	-	Nostoc punctiforme
soluble guanylate cyclase	-	Legionella pneumophila
soluble guanylate cyclase	-	Nostoc punctiforme

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
additional information	-	L2 H-NOX is a mixture of 5- and 6-coordinate complexes under room temperature conditions, at low temperature, it is primarily 6-coordinate, and at high temperature, there is a shift toward the 5-coordinate geometry	Legionella pneumophila

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Release 2023.1 (January 2023)