Activating Compound | Comment | Organism | Structure |
---|---|---|---|
BAY 41-2272 | one molecule is required for maximal enzyme activation and is tightly associated with sGC | Bos taurus |
Application | Comment | Organism |
---|---|---|
additional information | two nucleotide-binding sites with high and low affinity for GMP-CPP, one of the two sites constitutes the substrate site responsible for catalysis. The other site is the pseudosymmetric site, which exclusively serves as the binding site for YC-1 or BAY 41-2272 | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-dADP | mixture of competitive and noncompetitive inhibition, binding to the substrate site excludes the substrate GTP, whereas binding to the noncompetitive site has no effect on GTP binding, although the resulting complex is catalytically inactive. It binds to the high and low affinity sites with equivalent affinities. BAY 41-2272, which shares an analogous core structure to YC-1, fully inhibits 2'-dADP binding to the low affinity site, whereas the inhibition by YC-1 is incomplete | Bos taurus | |
adenosine 5'-beta,gamma-imido triphosphate | competitive inhibitor, reduces the Bmax for the binding of guanosine 5'-beta,gamma-methylene triphosphate, is tightly and selectively associated with the high affinity site | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bos taurus | |
Fe2+ | - |
Bos taurus | |
Mn2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Bos taurus | 3',5'-cyclic GMP + diphosphate | - |
? | |
guanosine 5'-beta,gamma-methylene triphosphate | - |
Bos taurus | ? | - |
? | |
additional information | 2'-deoxy-3'-GMP and cGMP exhibit no detectable affinity for the enzyme | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Bos taurus |
soluble guanylate cyclase | - |
Bos taurus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 27 | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
AMP-PNP | at 10°C | Bos taurus | |
0.015 | - |
AMP-PNP | at 27°C | Bos taurus |