Cloned (Comment) | Organism |
---|---|
epsilon2 endonuclease, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged wild-type and mutant enzyme | Candidatus Micrarchaeum acidiphilum |
Protein Variants | Comment | Organism |
---|---|---|
D357A | site-directed mutagenesis | Candidatus Micrarchaeum acidiphilum |
K224E | site-directed mutagenesis | Candidatus Micrarchaeum acidiphilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candidatus Micrarchaeum acidiphilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candidatus Micrarchaeum acidiphilum | C7DIA5 | ARMAN-2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | epsilon2 endonuclease cleaves both canonical and noncanonical bulge-helix-bulge motifs, BHB motif, both strict and relaxed | Candidatus Micrarchaeum acidiphilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | the enzyme consists of two duplicated catalytic units and one structural unit encoded on a single gene, representing a three-unit architecture, the conserved L10-pocket interaction between catalytic and structural unit is necessary for the assembly, protein domain structure, overview | Candidatus Micrarchaeum acidiphilum |
Synonyms | Comment | Organism |
---|---|---|
epsilon2 endonuclease | - |
Candidatus Micrarchaeum acidiphilum |
More | fourth type of archaeal tRNA splicing endonuclease | Candidatus Micrarchaeum acidiphilum |
three-unit tRNA splicing endonuclease | - |
Candidatus Micrarchaeum acidiphilum |
tRNA splicing endonuclease | - |
Candidatus Micrarchaeum acidiphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Candidatus Micrarchaeum acidiphilum |
General Information | Comment | Organism |
---|---|---|
evolution | in Archaea, three types of splicing endonuclease are produced, homotetrameric: alpha4, homodimeric: alpha2, and heterotetrameric: (alphabeta)2. epsilon2 endonuclease represents a fourth type: epsilon2. The presence of an epsilon2 endonuclease in an archaeon deeply branched within Euryarchaeota represents an example of the coevolution of tRNA and their processing enzymes. The ancestral alpha4 endonuclease is assumed to have undergone two independent gene duplication events giving rise to alpha2 and (alphabeta)2 architecture via subfunctionalization, while most of the (alphabeta)2 endonucleases and korarchaeal a2 endonuclease have acquired extra insertion sequences that interact with BHB motif to broaden their substrate specificity. Transition of splicing endonuclease from alpha4 to epsilon2 type, overview | Candidatus Micrarchaeum acidiphilum |
physiological function | tRNA splicing endonucleases are involved in the processing of pre-tRNA molecules | Candidatus Micrarchaeum acidiphilum |