BRENDA - Enzyme Database show
show all sequences of 4.6.1.12

Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis

Sando, T.; Takeno, S.; Watanabe, N.; Okumoto, H.; Kuzuyama, T.; Yamashita, A.; Hattori, M.; Ogasawara, N.; Fukusaki, E.; Kobayashi, A.; Biosci. Biotechnol. Biochem. 72, 2903-2917 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Hevea brasiliensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hevea brasiliensis
A9ZN12
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
latex
extremely low expression in latex
Hevea brasiliensis
-
leaf
highest expression in leaves
Hevea brasiliensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol
-
691382
Hevea brasiliensis
2-phospho-2C-methyl-D-erythritol 3,4-cyclophosphate + CMP
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Hevea brasiliensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
latex
extremely low expression in latex
Hevea brasiliensis
-
leaf
highest expression in leaves
Hevea brasiliensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol
-
691382
Hevea brasiliensis
2-phospho-2C-methyl-D-erythritol 3,4-cyclophosphate + CMP
-
-
-
?
Other publictions for EC 4.6.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747328
Liu
Crystal structure of IspF fro ...
Bacillus subtilis 168, Bacillus subtilis
Biosci. Rep.
38
BSR20171370
2018
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749009
Mandal
Enhanced production of stevio ...
Stevia rebaudiana
Plant Physiol. Biochem.
89
100-106
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
730270
Jung
-
Molecular and functional chara ...
Brassica rapa
J. Plant Biotechnol.
39
189-195
2012
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
730357
Kumar
2C-Methyl-D-erythritol 2,4-cyc ...
Stevia rebaudiana
Mol. Biol. Rep.
39
10971-10978
2012
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
3
-
-
3
-
-
730628
Zheng
-
Cloning and characterization o ...
Rauvolfia verticillata
Plant OMICS
4
82-90
2011
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
708124
Geist
Thiazolopyrimidine inhibitors ...
Arabidopsis thaliana, Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Plasmodium falciparum, Plasmodium falciparum NF54
ChemMedChem
5
1092-1101
2010
-
-
2
-
-
-
63
3
-
5
2
-
-
9
-
-
2
-
-
-
-
-
6
-
-
-
-
3
1
-
-
-
-
-
75
-
-
2
-
-
-
-
75
63
-
3
-
5
2
-
-
-
-
2
-
-
-
-
6
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
714711
Narayanasamy
Synthesis of 4-diphosphocytidy ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Chem. Biol.
17
117-122
2010
-
-
-
-
-
-
-
-
-
-
-
2
-
160
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
Theor. Appl. Genet.
118
653-669
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
709435
Ramsden
A structure-based approach to ...
Escherichia coli
J. Med. Chem.
52
2531-2542
2009
-
1
1
1
-
-
8
-
-
3
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
8
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
709487
Liu
-
A new 2-C-methyl-D-erythritol ...
Taxus x media
J. Med. Plant Res.
3
395-402
2009
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
4
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
1
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
691382
Sando
Cloning and characterization o ...
Hevea brasiliensis
Biosci. Biotechnol. Biochem.
72
2903-2917
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694098
Chen
Molecular cloning and characte ...
Cephalotaxus harringtonia
Mol. Biol. Rep.
36
1749-1756
2008
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
695176
Narayanasamy
Chemoenzymatic synthesis of 4- ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis Rv3582c
Tetrahedron Lett.
49
4461-4463
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680059
Baumgartner
-
Structure-based design and syn ...
Escherichia coli
Helv. Chim. Acta
90
1043-1068
2007
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682735
Calisto
Biosynthesis of isoprenoids in ...
Arabidopsis thaliana
Protein Sci.
16
2082-2088
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666594
Kim
-
Cloning and characterization o ...
Ginkgo biloba
Plant Cell Rep.
8
829-835
2006
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
672138
Lherbet
Absence of substrate channelin ...
Agrobacterium tumefaciens
Biochemistry
45
3548-3553
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675583
Illarionova
Nonmevalonate terpene biosynth ...
Plasmodium sp.
J. Org. Chem.
71
8824-8834
2006
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681826
Jin
-
Isolation and characterization ...
Taxus x media
Mol. Biol.
40
914-921
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
682464
Hsieh
Functional evidence for the in ...
Arabidopsis thaliana
Planta
223
779-784
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663548
Sgraja
A double mutation of Escherich ...
Escherichia coli
Acta Crystallogr. Sect. F
F61
625-629
2005
-
-
-
1
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654130
Ni
Structure of 2C-methyl-D-eryth ...
Shewanella oneidensis, Shewanella oneidensis MR1
Acta Crystallogr. Sect. D
D60
1949-1957
2004
-
-
1
1
-
-
-
-
-
2
1
-
-
6
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656263
Gabrielsen
Hexameric assembly of the bifu ...
Agrobacterium tumefaciens, Campylobacter jejuni, Escherichia coli
J. Biol. Chem.
279
52753-52761
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654081
Kishida
Structure and catalytic mechan ...
Thermus thermophilus
Acta Crystallogr. Sect. D
59
23-31
2003
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655848
Campbell
Characterization of the deplet ...
Escherichia coli
J. Bacteriol.
184
5609-5618
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656112
Richard
Structure and mechanism of 2-C ...
Escherichia coli
J. Biol. Chem.
277
8667-8672
2002
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656498
Steinbacher
Structure of 2C-methyl-D-eryth ...
Escherichia coli
J. Mol. Biol.
316
79-88
2002
-
-
-
1
-
-
-
-
-
2
-
1
-
8
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657196
Kemp
Structure of 2C-methyl-D-eryth ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
99
6591-6596
2002
-
-
-
1
-
-
-
-
-
2
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657313
Lehmann
Structure of 2C-methyl-D-eryth ...
Haemophilus influenzae
Proteins
49
135-138
2002
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655417
Rohdich
Biosynthesis of terpenoids. 2C ...
Plasmodium falciparum
Eur. J. Biochem.
268
3190-3197
2001
-
1
1
-
-
-
1
1
-
2
1
1
-
4
-
-
1
-
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
2
1
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
247122
Takagi
-
Studies on the nonmevalonate p ...
Escherichia coli
Tetrahedron Lett.
41
3395-3398
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657177
Herz
Biosynthesis of terpenoids: Yg ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
97
2486-2490
2000
-
-
-
-
-
-
-
-
-
2
1
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-