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Literature summary for 4.5.1.3 extracted from

  • Stourman, N.V.; Rose, J.H.; Vuilleumier, S.; Armstrong, R.N.
    Catalytic mechanism of dichloromethane dehalogenase from Methylophilus sp. strain DM11 (2003), Biochemistry, 42, 11048-11056.
    View publication on PubMed
Search for more literature for 4.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
glutathione
-
 Methylophilus sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methylophilus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00162
-
 CH2BrCl 25°C, pH 8 Methylophilus sp.
0.0017
-
 Diiodomethane 25°C, pH 8 Methylophilus sp.
0.002
-
 CH2ClI 25°C, pH 8 Methylophilus sp.
0.0031
-
 Dibromomethane 25°C, pH 8 Methylophilus sp.
0.031
-
 Dichloromethane 25°C, pH 8 Methylophilus sp.
0.039
-
 Iodoethane 25°C, pH 8 Methylophilus sp.
0.053
-
 CH2ClF 25°C, pH 8 Methylophilus sp.
0.33
-
 chloroethane 25°C, pH 8 Methylophilus sp.
0.33
-
 Bromoethane 25°C, pH 8 Methylophilus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

Organism UniProt Comment Textmining
Methylophilus sp. P43387
-
-
Methylophilus sp. DM11 P43387
-
-

Purification (Commentary)

Purification (Comment) Organism
strain DM11 Methylophilus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bromoethane + H2O
-
 Methylophilus sp. ethanal + Br-
-
 ?
bromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
CH2BrCl + H2O
-
 Methylophilus sp. formaldehyde + Br- + Cl-
-
 ?
CH2BrCl + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br- + Cl-
-
 ?
CH2ClF + H2O
-
 Methylophilus sp. formaldehyde + Cl- + F-
-
 ?
CH2ClF + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl- + F-
-
 ?
CH2ClI + H2O
-
 Methylophilus sp. formaldehyde + Cl- + I-
-
 ?
chloroethane + H2O
-
 Methylophilus sp. ethanal + Cl-
-
 ?
dibromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
dibromomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?
diiodomethane + H2O
-
 Methylophilus sp. formaldehyde + I-
-
 ?
diiodomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + I-
-
 ?
iodoethane + H2O
-
 Methylophilus sp. ethanal + I-
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 CH2ClF 25°C, pH 8 Methylophilus sp.
0.1
-
 chloroethane 25°C, pH 8 Methylophilus sp.
0.14
-
 Bromomethane 25°C, pH 8 Methylophilus sp.
0.15
-
 Iodoethane 25°C, pH 8 Methylophilus sp.
1
-
 CH2BrCl 25°C, pH 8 Methylophilus sp.
1.03
-
 CH2ClI 25°C, pH 8 Methylophilus sp.
1.31
-
 Diiodomethane 25°C, pH 8 Methylophilus sp.
1.51
-
 Dichloromethane 25°C, pH 8 Methylophilus sp.
3.18
-
 Dibromomethane 25°C, pH 8 Methylophilus sp.