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Literature summary for 4.4.1.30 extracted from

  • Zhou, W.; Ding, W.L.; Zeng, X.L.; Dong, L.L.; Zhao, B.; Zhou, M.; Scheer, H.; Zhao, K.H.; Yang, X.
    Structure and mechanism of the phycobiliprotein lyase CpcT (2014), J. Biol. Chem., 289, 26677-26689.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.30

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Nostoc sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the apoenzyme and its complex with phycocyanobilin at 1.95 and 2.50 A resolution Nostoc sp.

Protein Variants

Protein Variants Comment Organism
D163A lyase activity is less than 5% compared to wild-type enzyme Nostoc sp.
D163V lyase activity is less than 5% compared to wild-type enzyme Nostoc sp.
D63N lyase activity is about 70% compared to wild-type enzyme Nostoc sp.
E111Q lyase activity is about 75% compared to wild-type enzyme Nostoc sp.
K87M lyase activity is about 70% compared to wild-type enzyme Nostoc sp.
M118A lyase activity is about 25% compared to wild-type enzyme Nostoc sp.
N83A lyase activity is about 35% compared to wild-type enzyme Nostoc sp.
Q55A lyase activity is about 30% compared to wild-type enzyme Nostoc sp.
R141I lyase activity is about 15% compared to wild-type enzyme Nostoc sp.
R68A lyase activity is about 10% compared to wild-type enzyme Nostoc sp.
S150A lyase activity is about 125% compared to wild-type enzyme Nostoc sp.
S161V lyase activity is about 105% compared to wild-type enzyme Nostoc sp.
Y59A lyase activity is about 35% compared to wild-type enzyme Nostoc sp.
Y65F lyase activity is less than 5% compared to wild-type enzyme Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc sp. Q8YLF9
-
-
Nostoc sp. PCC 7120 Q8YLF9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nostoc sp.

Subunits

Subunits Comment Organism
dimer at the concentration of 0.003 mM, the enzyme is mostly monomeric in the absence of phycocyanobilin, but it tends to dimerize in the presence of 0.006 mM phycocyanobilin Nostoc sp.
monomer at the concentration of 0.003 mM, the enzyme is mostly monomeric in the absence of phycocyanobilin, but it tends to dimerize in the presence of 0.006 mM phycocyanobilin Nostoc sp.

Synonyms

Synonyms Comment Organism
phycobiliprotein lyase CpcT
-
 Nostoc sp.