Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in Pichia pastoris strain KM71H | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in apoform or in complex with either one of three product analogues, S-hexyl-, 4-phenyl-butyl-, and 2-hydroxy-4-phenyl-butyl-glutathione, sitting drop vapor diffusion, mixing of 0.001 ml of 3.5 mg/ml protein solution containing 1 mM GSH with 0.001 ml of reservoir solution containing 1.8-2.2 M NH4SO4, 0.2 M NaCl, and 0.1 M sodium cacodylate, pH 6.1-6.8, 1-4 days, room temperature, soaking in 1 mM ligand solutions, X-ray diffraction structure determination and analysis at 2.4-3.2 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W116A | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Homo sapiens |
W116F | site-directed mutagenesis, the mutant shows a 3fold increased turnover of leukotriene A4 compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
0.04 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant mutant W116A | Homo sapiens | |
0.095 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant mutant W116F | Homo sapiens | |
0.3 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
1.42 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant mutant W116A | Homo sapiens | |
2.38 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant mutant W116F | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | integral membrane protein in the outer leaflet of the nuclear envelope and in the endoplasmic reticulum | Homo sapiens | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + glutathione | Homo sapiens | - |
leukotriene C4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16873 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + glutathione | - |
Homo sapiens | leukotriene C4 | - |
? | |
leukotriene A4 + glutathione | glutathione-enzyme binding structure, overview | Homo sapiens | leukotriene C4 | i.e. (5S)-hydroxy-(6R)-S-glutathionyl-7,9-trans-11,14-cis-eicosatetraenoic acid | ? |
Synonyms | Comment | Organism |
---|---|---|
leukotriene C4 synthase | - |
Homo sapiens |
LT C4 synthase | - |
Homo sapiens |
LTC4S | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
16.8 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant mutant W116A | Homo sapiens | |
17.7 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant mutant W116F | Homo sapiens | |
26 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
32 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant mutant W116A | Homo sapiens | |
74.3 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant mutant W116F | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the superfamily of membrane-associated proteins in eicosanoid and glutathione metabolism, which consists of six integral membrane proteins | Homo sapiens |
additional information | enzyme residue Trp116 is not essential for catalysis but helps position the omega-end of leukotriene A4 | Homo sapiens |
physiological function | the enzyme catalyzes the conjugation reaction between the fatty acid leukotriene A4 and GSH to form the pro-inflammatory leukotriene C4, an important mediator of asthma | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant mutant W116F | Homo sapiens | |
11 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant mutant W116A | Homo sapiens | |
40 | - |
leukotriene A4 | pH 7.8, temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens | |
730 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant mutant W116F | Homo sapiens | |
810 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant mutant W116A | Homo sapiens | |
870 | - |
glutathione | pH 7.8, temperature not specified in the publication, recombinant wild-type enzyme | Homo sapiens |