BRENDA - Enzyme Database show
show all sequences of 4.4.1.19

Identification of coenzyme M biosynthetic phosphosulfolactate synthase: a new family of sulfonate-biosynthesizing enzymes

Graham, D.E.; Xu, H.; White, R.H.; J. Biol. Chem. 277, 13421-13429 (2002)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
K137N
no measurable enzymatic activity
Methanocaldococcus jannaschii
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Methanocaldococcus jannaschii
LiCl
weak
Methanocaldococcus jannaschii
MgCl2
inhibition above 10 mM
Methanocaldococcus jannaschii
additional information
further weak inhibitors, not inhibitory: K+, Na+, NH4+
Methanocaldococcus jannaschii
phosphoenolpyruvate
substrate inhibition above 5 mM
Methanocaldococcus jannaschii
sulfite
substrate inhibition above 3 mM
Methanocaldococcus jannaschii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
9.8
-
phosphoenolpyruvate
-
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Methanocaldococcus jannaschii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
32000
-
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
86700
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Methanocaldococcus jannaschii
catalyzes the first step in coenzyme M biosynthesis
?
-
-
-
sulfite + phosphoenolpyruvate
Methanocaldococcus jannaschii
-
(2R)-O-phospho-3-sulfolactate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
Q57703
-
-
Reaction
Reaction
Commentary
Organism
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + sulfite
catalyses the Michael addition of sulfite to phosphoenolpyruvate, requires Mg2+, specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite
Methanocaldococcus jannaschii
Renatured (Commentary)
Commentary
Organism
5 mM MgCl2 restores activity of EDTA-treated enzyme
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4
-
recombinant enzyme
Methanocaldococcus jannaschii
Storage Stability
Storage Stability
Organism
4░C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
catalyzes the first step in coenzyme M biosynthesis
639025
Methanocaldococcus jannaschii
?
-
-
-
-
sulfite + phosphoenolpyruvate
-
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
-
-
-
-
sulfite + phosphoenolpyruvate
-
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
enantiomeric ratio of 84:16 R-isomer:S-isomer
639025
Methanocaldococcus jannaschii
ir
Subunits
Subunits
Commentary
Organism
trimer
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Methanocaldococcus jannaschii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
-
Methanocaldococcus jannaschii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
phosphoenolpyruvate
-
Methanocaldococcus jannaschii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K137N
no measurable enzymatic activity
Methanocaldococcus jannaschii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Methanocaldococcus jannaschii
LiCl
weak
Methanocaldococcus jannaschii
MgCl2
inhibition above 10 mM
Methanocaldococcus jannaschii
additional information
further weak inhibitors, not inhibitory: K+, Na+, NH4+
Methanocaldococcus jannaschii
phosphoenolpyruvate
substrate inhibition above 5 mM
Methanocaldococcus jannaschii
sulfite
substrate inhibition above 3 mM
Methanocaldococcus jannaschii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.9
-
phosphoenolpyruvate
-
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
9.8
-
phosphoenolpyruvate
-
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
32000
-
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
86700
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Methanocaldococcus jannaschii
catalyzes the first step in coenzyme M biosynthesis
?
-
-
-
sulfite + phosphoenolpyruvate
Methanocaldococcus jannaschii
-
(2R)-O-phospho-3-sulfolactate
-
-
-
Renatured (Commentary) (protein specific)
Commentary
Organism
5 mM MgCl2 restores activity of EDTA-treated enzyme
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4
-
recombinant enzyme
Methanocaldococcus jannaschii
Storage Stability (protein specific)
Storage Stability
Organism
4░C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
catalyzes the first step in coenzyme M biosynthesis
639025
Methanocaldococcus jannaschii
?
-
-
-
-
sulfite + phosphoenolpyruvate
-
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
-
-
-
-
sulfite + phosphoenolpyruvate
-
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
enantiomeric ratio of 84:16 R-isomer:S-isomer
639025
Methanocaldococcus jannaschii
ir
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Methanocaldococcus jannaschii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
-
Methanocaldococcus jannaschii
Other publictions for EC 4.4.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748234
Partovi
Coenzyme M biosynthesis in ba ...
Xanthobacter autotrophicus
J. Biol. Chem.
293
5236-5246
2018
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1
1
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1
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5
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1
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1
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3
-
1
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1
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1
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1
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1
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1
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1
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3
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1
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1
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-
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1
1
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678954
Liu
-
Hsa32, a phosphosulfolactate s ...
Arabidopsis thaliana
Bot. Stud.
47
389-395
2006
-
1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
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-
639026
Wise
The structural determination o ...
Methanocaldococcus jannaschii
J. Biol. Chem.
278
45858-45863
2003
-
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-
1
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2
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1
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1
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1
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639025
Graham
Identification of coenzyme M b ...
Methanocaldococcus jannaschii
J. Biol. Chem.
277
13421-13429
2002
-
-
-
-
1
-
6
1
-
1
3
2
-
2
-
-
-
1
1
-
1
1
3
1
-
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-
1
1
-
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1
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-
-
-
-
1
-
-
6
1
1
-
1
3
2
-
-
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-
1
-
1
1
3
1
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1
1
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