Data extracted from this reference:
Engineering
K137N
no measurable enzymatic activity
Methanocaldococcus jannaschii
Inhibitors
EDTA
Methanocaldococcus jannaschii
LiCl
weak
Methanocaldococcus jannaschii
MgCl2
inhibition above 10 mM
Methanocaldococcus jannaschii
additional information
further weak inhibitors, not inhibitory: K+, Na+, NH4+
Methanocaldococcus jannaschii
phosphoenolpyruvate
substrate inhibition above 5 mM
Methanocaldococcus jannaschii
sulfite
substrate inhibition above 3 mM
Methanocaldococcus jannaschii
KM Value [mM]
9.8
phosphoenolpyruvate
Methanocaldococcus jannaschii
Metals/Ions
Mg2+
required
Methanocaldococcus jannaschii
Molecular Weight [Da]
28000
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
32000
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
86700
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
additional information
Methanocaldococcus jannaschii
catalyzes the first step in coenzyme M biosynthesis
?
sulfite + phosphoenolpyruvate
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
Organism
Methanocaldococcus jannaschii
Q57703
Reaction
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + sulfite
catalyses the Michael addition of sulfite to phosphoenolpyruvate, requires Mg2+, specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite
Methanocaldococcus jannaschii
Renatured (Commentary)
5 mM MgCl2 restores activity of EDTA-treated enzyme
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg]
2.4
recombinant enzyme
Methanocaldococcus jannaschii
Storage Stability
4°C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
additional information
catalyzes the first step in coenzyme M biosynthesis
639025
Methanocaldococcus jannaschii
?
sulfite + phosphoenolpyruvate
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
sulfite + phosphoenolpyruvate
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
enantiomeric ratio of 84:16 R-isomer:S-isomer
639025
Methanocaldococcus jannaschii
ir
Subunits
trimer
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
pH Optimum
8.5
Methanocaldococcus jannaschii
pH Range
6
11
Methanocaldococcus jannaschii
Ki Value [mM]
1.9
phosphoenolpyruvate
Methanocaldococcus jannaschii
Engineering (protein specific)
K137N
no measurable enzymatic activity
Methanocaldococcus jannaschii
Inhibitors (protein specific)
EDTA
Methanocaldococcus jannaschii
LiCl
weak
Methanocaldococcus jannaschii
MgCl2
inhibition above 10 mM
Methanocaldococcus jannaschii
additional information
further weak inhibitors, not inhibitory: K+, Na+, NH4+
Methanocaldococcus jannaschii
phosphoenolpyruvate
substrate inhibition above 5 mM
Methanocaldococcus jannaschii
sulfite
substrate inhibition above 3 mM
Methanocaldococcus jannaschii
Ki Value [mM] (protein specific)
1.9
phosphoenolpyruvate
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
9.8
phosphoenolpyruvate
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Mg2+
required
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
28000
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
32000
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
86700
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
additional information
Methanocaldococcus jannaschii
catalyzes the first step in coenzyme M biosynthesis
?
sulfite + phosphoenolpyruvate
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
Renatured (Commentary) (protein specific)
5 mM MgCl2 restores activity of EDTA-treated enzyme
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg] (protein specific)
2.4
recombinant enzyme
Methanocaldococcus jannaschii
Storage Stability (protein specific)
4°C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
additional information
catalyzes the first step in coenzyme M biosynthesis
639025
Methanocaldococcus jannaschii
?
sulfite + phosphoenolpyruvate
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
sulfite + phosphoenolpyruvate
639025
Methanocaldococcus jannaschii
(2R)-O-phospho-3-sulfolactate
enantiomeric ratio of 84:16 R-isomer:S-isomer
639025
Methanocaldococcus jannaschii
ir
Subunits (protein specific)
trimer
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Methanocaldococcus jannaschii
pH Optimum (protein specific)
8.5
Methanocaldococcus jannaschii
pH Range (protein specific)
6
11
Methanocaldococcus jannaschii
Other publictions for EC 4.4.1.19
748234
Partovi
Coenzyme M biosynthesis in ba ...
Xanthobacter autotrophicus
J. Biol. Chem.
293
5236-5246
2018
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678954
Liu
-
Hsa32, a phosphosulfolactate s ...
Arabidopsis thaliana
Bot. Stud.
47
389-395
2006
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639026
Wise
The structural determination o ...
Methanocaldococcus jannaschii
J. Biol. Chem.
278
45858-45863
2003
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639025
Graham
Identification of coenzyme M b ...
Methanocaldococcus jannaschii
J. Biol. Chem.
277
13421-13429
2002
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