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Literature summary for 4.4.1.1 extracted from

  • Lee, Z.W.; Low, Y.L.; Huang, S.; Wang, T.; Deng, L.W.
    The cystathionine beta-lyase/hydrogen sulfide system maintains cellular glutathione status (2014), Biochem. J., 460, 425-435.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expressionin HEK-293 cells, Hep-G2 cells and IMR-90 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E339A site-directed mutagenesis, the hyperactive mutant displays a 4fold greater H2S production compared to the wild-type enzyme Homo sapiens
E349A site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme Homo sapiens
additional information enzyme silencing by CSE-specific siRNA in HEK-293 cells, Hep-G2 cells, and IMR90 cells with loss of the protein band to at least 90% efficacy in the siRNA-transfected samples as compared with the scrambled-siRNA negative control Homo sapiens
Y114A site-directed mutagenesis Homo sapiens
Y60A site-directed mutagenesis, inactive mutant, which cannot provide antioxidative activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
propargylglycine
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P32929
-
-

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CSE
-
Homo sapiens

General Information

General Information Comment Organism
malfunction inhibiting the enzyme activity with propargylglycine or silencing CSE expression using an siRNA approach results in a greater reduction in cell viability under exposure to the oxidizing agent hydrogen peroxide. Cellular oxidative stress also increases significantly upon propargylglycine inhibition or enzyme knockdown. Increased sensitivity towards H2O2-induced cytotoxicity in CSE-siRNAtransfected cells is associated with a decreased glutathione concentration (GSH) and glutathione ratio (GSH/GSSG). Incubation of cells with exogenous H2S increases the GSH concentration and GSH/GSSG ratio Homo sapiens
physiological function cystathionine gamma-lyase is an important enzyme responsible for endogenous H2S production in mammalian systems. Modulation of enzyme catalytic activity alters its antioxidative activity. Role of CSE in contributing to cellular antioxidative mechanisms in cell lines of peripheral tissue origin. Knockdown of CSE does not result in significant spontaneous cell death, indicating that CSE is not vital for cell survival in the cell lines studied Homo sapiens