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Literature summary for 4.4.1.1 extracted from

  • Knoll, C.; du Toit, M.; Schnell, S.; Rauhut, D.; Irmler, S.
    Cloning and characterisation of a cystathionine beta/gamma-lyase from two Oenococcus oeni oenological strains (2011), Appl. Microbiol. Biotechnol., 89, 1051-1060.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oenococcus oeni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
L-Cys pH 8.0, 37°C Oenococcus oeni
0.069
-
L-Cys pH 8.0, 37°C Oenococcus oeni
0.232
-
L-cystathionine pH 8.0, 37°C Oenococcus oeni
0.236
-
L-cystathionine pH 8.0, 37°C Oenococcus oeni
1.153
-
(DL)-homocysteine pH 8.0, 37°C Oenococcus oeni
1.417
-
(DL)-homocysteine pH 8.0, 37°C Oenococcus oeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE, x * 41500, calculated Oenococcus oeni
41500
-
x * 40000, SDS-PAGE, x * 41500, calculated Oenococcus oeni

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol not inhibitory up to 15% v/v Oenococcus oeni

Organism

Organism UniProt Comment Textmining
Oenococcus oeni
-
-
-
Oenococcus oeni R1105
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(DL)-homocysteine
-
Oenococcus oeni H2S + ?
-
?
(DL)-homocysteine
-
Oenococcus oeni R1105 H2S + ?
-
?
L-Cys
-
Oenococcus oeni H2S + NH3 + pyruvate
-
?
L-Cys
-
Oenococcus oeni R1105 H2S + NH3 + pyruvate
-
?
L-cystathionine + H2O
-
Oenococcus oeni L-cysteine + 2-oxobutanoate + NH3
-
?
L-cystathionine + H2O
-
Oenococcus oeni R1105 L-cysteine + 2-oxobutanoate + NH3
-
?
L-cystathionine + H2O
-
Oenococcus oeni L-homocysteine + pyruvate + NH3
-
?
L-cystathionine + H2O
-
Oenococcus oeni R1105 L-homocysteine + pyruvate + NH3
-
?
additional information enzyme is able to demethiolate methionine into methanethiol Oenococcus oeni ?
-
?
additional information enzyme is able to demethiolate methionine into methanethiol Oenococcus oeni R1105 ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE, x * 41500, calculated Oenococcus oeni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
stable up to Oenococcus oeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
substrate cystathionine Oenococcus oeni

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
substrate cystathionine, no activity below Oenococcus oeni