Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Oenococcus oeni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
L-Cys | pH 8.0, 37°C | Oenococcus oeni | |
0.069 | - |
L-Cys | pH 8.0, 37°C | Oenococcus oeni | |
0.232 | - |
L-cystathionine | pH 8.0, 37°C | Oenococcus oeni | |
0.236 | - |
L-cystathionine | pH 8.0, 37°C | Oenococcus oeni | |
1.153 | - |
(DL)-homocysteine | pH 8.0, 37°C | Oenococcus oeni | |
1.417 | - |
(DL)-homocysteine | pH 8.0, 37°C | Oenococcus oeni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE, x * 41500, calculated | Oenococcus oeni |
41500 | - |
x * 40000, SDS-PAGE, x * 41500, calculated | Oenococcus oeni |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | not inhibitory up to 15% v/v | Oenococcus oeni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oenococcus oeni | - |
- |
- |
Oenococcus oeni R1105 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(DL)-homocysteine | - |
Oenococcus oeni | H2S + ? | - |
? | |
(DL)-homocysteine | - |
Oenococcus oeni R1105 | H2S + ? | - |
? | |
L-Cys | - |
Oenococcus oeni | H2S + NH3 + pyruvate | - |
? | |
L-Cys | - |
Oenococcus oeni R1105 | H2S + NH3 + pyruvate | - |
? | |
L-cystathionine + H2O | - |
Oenococcus oeni | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cystathionine + H2O | - |
Oenococcus oeni R1105 | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cystathionine + H2O | - |
Oenococcus oeni | L-homocysteine + pyruvate + NH3 | - |
? | |
L-cystathionine + H2O | - |
Oenococcus oeni R1105 | L-homocysteine + pyruvate + NH3 | - |
? | |
additional information | enzyme is able to demethiolate methionine into methanethiol | Oenococcus oeni | ? | - |
? | |
additional information | enzyme is able to demethiolate methionine into methanethiol | Oenococcus oeni R1105 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE, x * 41500, calculated | Oenococcus oeni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
stable up to | Oenococcus oeni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
substrate cystathionine | Oenococcus oeni |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
substrate cystathionine, no activity below | Oenococcus oeni |