Literature summary for 4.3.99.5 extracted from

Katsuyama, Y.; Sato, Y.; Sugai, Y.; Higashiyama, Y.; Senda, M.; Senda, T.; Ohnishi, Y.
Crystal structure of the nitrosuccinate lyase CreD in complex with fumarate provides insights into the catalytic mechanism for nitrous acid elimination (2018), FEBS J., 285, 1540-1555 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptomyces cremeus

Crystallization (Commentary)

Crystallization (Comment)	Organism
free enzyme and enzyme in complex with fumarate, using 0.1 M Tris/HCl pH 8.2-8.5 and 1.8-2.0 M ammonium sulfate	Streptomyces cremeus

Protein Variants

Protein Variants	Comment	Organism
D109N	the mutant loses the activity to produce nitrite almost completely	Streptomyces cremeus
D109V	the mutant loses the activity to produce nitrite almost completely	Streptomyces cremeus
H237A	the mutant loses the activity to produce nitrite completely	Streptomyces cremeus
K292A	the mutant loses the activity to produce nitrite completely	Streptomyces cremeus
N294A	the mutant loses the activity to produce nitrite almost completely	Streptomyces cremeus
N77A	the mutation slightly weakens the nitrite production compared to the wild type enzyme	Streptomyces cremeus
R325A	the mutant loses the activity to produce nitrite completely	Streptomyces cremeus
S286A	the mutant loses the activity to produce nitrite almost completely	Streptomyces cremeus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
203000	-	calculated from amino acid sequence	Streptomyces cremeus
228500	-	gel filtration with inline multiangle light scattering	Streptomyces cremeus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-nitrobutanedioate	Streptomyces cremeus	-	fumarate + nitrite	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces cremeus	A0A0K2JL82	-	-

Purification (Commentary)

Purification (Comment)	Organism
His60 Ni Superflow resin column chromatography and Resource Q column chromatography	Streptomyces cremeus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrobutanedioate	-	Streptomyces cremeus	fumarate + nitrite	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Streptomyces cremeus

Synonyms

Synonyms	Comment	Organism
creD	-	Streptomyces cremeus

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Release 2023.1 (January 2023)