BRENDA - Enzyme Database show
show all sequences of 4.3.3.1

Microbial degradation of validamycin A by Flavobacterium saccharophilum. Enzymatic cleavage of C-N linkage in validoxylamine A

Asano, N.; Takeuchi, M.; Ninomiya, K.;Kameda, Y.; Matsui, K.; J. Antibiot. 37, 859-867 (1984)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Flavobacterium saccharophilum
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium saccharophilum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-4-nitrophenyl-3-ketovalidamine
synthetic model compound
648708
Flavobacterium saccharophilum
4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one
-
648708
Flavobacterium saccharophilum
?
N-4-nitrophenyl-3-ketovalienamine
synthetic model compound
648708
Flavobacterium saccharophilum
4-nitroaniline + ?
-
648708
Flavobacterium saccharophilum
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Flavobacterium saccharophilum
16020
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-4-nitrophenyl-3-ketovalidamine
synthetic model compound
648708
Flavobacterium saccharophilum
4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one
-
648708
Flavobacterium saccharophilum
?
N-4-nitrophenyl-3-ketovalienamine
synthetic model compound
648708
Flavobacterium saccharophilum
4-nitroaniline + ?
-
648708
Flavobacterium saccharophilum
?
Other publictions for EC 4.3.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714560
Kim
-
Gene cloning and expression of ...
Flavobacterium saccharophilum, Flavobacterium saccharophilum IFO 13984
Biotechnol. Bioprocess Eng.
16
366-373
2011
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701773
Zhang
Purification and Characterizat ...
Stenotrophomonas maltophilia
Appl. Biochem. Biotechnol.
162
966-74
2010
-
-
-
-
-
-
3
1
-
2
1
-
-
2
-
-
1
-
-
1
1
-
1
-
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
3
-
1
-
2
1
-
-
-
-
1
-
1
1
-
1
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
701595
Zhang
-
Study on optimal production of ...
Stenotrophomonas maltophilia
Afr. J. Biotechnol.
8
5482-5488
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
677731
Zhang
Preparation of 3-ketovalidoxyl ...
Stenotrophomonas maltophilia
Appl. Microbiol. Biotechnol.
73
1275-1281
2007
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648712
Takeuchi
Chemical modification by 2,4,6 ...
Flavobacterium saccharophilum
Chem. Pharm. Bull.
38
1419-1420
1990
-
-
-
-
-
-
1
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
648711
Takeuchi
Fluorometric studies on the ro ...
Flavobacterium saccharophilum
Chem. Pharm. Bull.
36
3540-3545
1988
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648710
Takeuchi
Chemical modification by dieth ...
Flavobacterium saccharophilum
J. Biochem.
99
1571-1577
1986
-
-
-
-
-
-
1
-
-
1
2
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
2
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648709
Takeuchi
Purification and properties of ...
Flavobacterium saccharophilum
J. Biochem.
98
1631-1638
1985
-
-
-
-
-
1
9
3
2
1
1
-
-
2
-
-
1
-
-
1
1
1
7
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
3
2
1
1
-
-
-
-
1
-
1
1
1
7
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
648708
Asano
Microbial degradation of valid ...
Flavobacterium saccharophilum
J. Antibiot.
37
859-867
1984
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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