Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21-DE3 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C441A | striking decrease in the enzymatic activity, while retaining the overall secondary to quaternary structure of the protein | Mycobacterium tuberculosis |
C441S | significant increase in activity, as compared to the wild-type enzyme, the mutant enzyme has higher thermal stability and maintains significant activity at high temperatures. The mutant shows a marked increase in enzymatic activity as it lacks this cysteine dependent feedback inhibition | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fumarate | a potential feedback mechanism is proposed, wherein the Cys441 is covalently modified to S-(2-succinyl) cysteine by one of the products, fumarate | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
2-(Nomega-L-arginino)succinate | pH 7.4, 25°C, mutant enzyme C441S | Mycobacterium tuberculosis | |
0.112 | - |
2-(Nomega-L-arginino)succinate | pH 7.4, 25°C, wild-type enzyme | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
non-denaturing PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(Nomega-L-arginino)succinate | Mycobacterium tuberculosis | last enzyme of the arginine biosynthesis pathway | fumarate + L-arginine | - |
? | |
2-(Nomega-L-arginino)succinate | Mycobacterium tuberculosis ATCC 25618 | last enzyme of the arginine biosynthesis pathway | fumarate + L-arginine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPY7 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WPY7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(Nomega-L-arginino)succinate | - |
Mycobacterium tuberculosis | fumarate + L-arginine | - |
? | |
2-(Nomega-L-arginino)succinate | last enzyme of the arginine biosynthesis pathway | Mycobacterium tuberculosis | fumarate + L-arginine | - |
? | |
2-(Nomega-L-arginino)succinate | - |
Mycobacterium tuberculosis ATCC 25618 | fumarate + L-arginine | - |
? | |
2-(Nomega-L-arginino)succinate | last enzyme of the arginine biosynthesis pathway | Mycobacterium tuberculosis ATCC 25618 | fumarate + L-arginine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50900 | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ArgH | - |
Mycobacterium tuberculosis |
Rv1659 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42.5 | - |
Tm-value of mutant enzyme C441A | Mycobacterium tuberculosis |
47 | - |
Tm-value of wild-type enzyme | Mycobacterium tuberculosis |
53 | - |
Tm-value of mutant enzyme C441S | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.63 | - |
2-(Nomega-L-arginino)succinate | pH 7.4, 25°C, wild-type enzyme | Mycobacterium tuberculosis | |
29.27 | - |
2-(Nomega-L-arginino)succinate | pH 7.4, 25°C, mutant enzyme C441S | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | last enzyme of the arginine biosynthesis pathway. Esential enzyme for the growth and survival of Mycobacterium tuberculosis | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
2-(Nomega-L-arginino)succinate | pH 7.4, 25°C, wild-type enzyme | Mycobacterium tuberculosis | |
914 | - |
2-(Nomega-L-arginino)succinate | pH 7.4, 25°C, mutant enzyme C441S | Mycobacterium tuberculosis |