Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-valine | activator, (0-10 mM) leads to a nearly 50% increase in MsIlvA activity | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
K67A | inactive mutant enzyme, the mutant enzyme is not yellow, as observed for the wild-type enzyme | Mycolicibacterium smegmatis |
K67Q | inactive mutant enzyme, the mutant enzyme is not yellow, as observed for the wild-type enzyme | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Cycloserine | powerful suicide inactivator that generates a covalent pyridoxal 5'-phosphate-isoxazole complex | Mycolicibacterium smegmatis | |
L-isoleucine | less than 2% activity remains after addition of 0.5 mM L-isoleucine | Mycolicibacterium smegmatis | |
L-valine | - |
Mycolicibacterium smegmatis | |
Mn2+ | 50 mM, 50% loss of activity | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.66 | - |
L-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
2.86 | - |
L-allo-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
3.01 | - |
beta-chloro-L-alanine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
106.3 | - |
L-serine | pH 8.0, 30°C | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50 mM, 1.7fold activation | Mycolicibacterium smegmatis | |
K+ | 50 mM, 1.5fold activation | Mycolicibacterium smegmatis | |
Li+ | 50 mM, 1.5fold activation | Mycolicibacterium smegmatis | |
Mg2+ | 50 mM, 1.6fold activation | Mycolicibacterium smegmatis | |
Na+ | 50 mM, 1.5fold activation | Mycolicibacterium smegmatis | |
NH4+ | 50 mM, 1.75fold activation | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine | Mycolicibacterium smegmatis | the enzyme is involved in the biosynthesis of L-isoleucine | 2-oxobutanoate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QX60 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloro-L-alanine | - |
Mycolicibacterium smegmatis | 3-chloro-2-oxopropionate + NH3 | - |
? | |
L-allo-threonine | - |
Mycolicibacterium smegmatis | ? | - |
? | |
L-serine | - |
Mycolicibacterium smegmatis | pyruvate + NH3 | - |
? | |
L-threonine | - |
Mycolicibacterium smegmatis | 2-oxobutanoate + NH3 | - |
? | |
L-threonine | the enzyme is involved in the biosynthesis of L-isoleucine | Mycolicibacterium smegmatis | 2-oxobutanoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
ilvA | - |
Mycolicibacterium smegmatis |
MSMEG3183 | - |
Mycolicibacterium smegmatis |
Threonine deaminase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.69 | - |
L-allo-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
9.42 | - |
L-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
27.15 | - |
beta-chloro-L-alanine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
32.59 | - |
L-serine | pH 8.0, 30°C | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.9 | pH has no significant effect on activity | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of L-isoleucine | Mycolicibacterium smegmatis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-serine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
1.99 | - |
L-allo-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
5.7 | - |
L-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
9.02 | - |
beta-chloro-L-alanine | pH 8.0, 30°C | Mycolicibacterium smegmatis |