Data extracted from this reference:
Inhibitors
additional information
Pseudomonas sp.
KM Value [mM]
0.14
D-serine O-sulfate
enzyme B
Pseudomonas sp.
0.17
D-Cystine
enzyme B
Pseudomonas sp.
1.1
S-Benzyl-L-cysteine
enzyme A
Pseudomonas sp.
1.3
O-benzyl-L-serine
enzyme A
Pseudomonas sp.
2
L-cystine
enzyme A
Pseudomonas sp.
2
O-benzyl-DL-serine
enzyme B, calculated on the basis of the non-reactivity of the L-isomer
Pseudomonas sp.
2.2
D-cystathionine
enzyme B
Pseudomonas sp.
5.4
L-serine O-sulfate
enzyme B
Pseudomonas sp.
7.9
L-serine O-sulfate
enzyme A
Pseudomonas sp.
8.3
D-serine
enzyme B
Pseudomonas sp.
8.5
L-cystathionine
enzyme B
Pseudomonas sp.
9.1
L-cystathionine
enzyme A
Pseudomonas sp.
17.8
S-methyl-L-cysteine
enzyme A
Pseudomonas sp.
20
O-acetyl-L-serine
enzyme A
Pseudomonas sp.
38
S-ethyl-L-cysteine
enzyme A
Pseudomonas sp.
56
S-methyl-L-cysteine
enzyme B
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
additional information
Pseudomonas sp.
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
additional information
Pseudomonas sp. FR
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
S-methyl-L-cysteine
Pseudomonas sp.
growth pattern is similar to that observed with L-serine O-sulfate
?
Organism
Purification (Commentary)
Specific Activity [micromol/min/mg]
additional information
Pseudomonas sp.
Substrates and Products (Substrate)
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp.
?
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp. FR
?
3-cyano-L-alanine
6035
Pseudomonas sp.
?
beta-ureido-alpha-aminopropionic acid
6035
Pseudomonas sp.
?
D-cystathionine
6035
Pseudomonas sp.
?
D-cysteine
6035
Pseudomonas sp.
?
D-serine
6035
Pseudomonas sp.
?
D-serine-O-sulfate
6035
Pseudomonas sp.
pyruvate + NH3 + SO42-
DL-cysteine
6035
Pseudomonas sp.
?
DL-lanthionine
6035
Pseudomonas sp.
?
L-cystathionine + H2O
6035
Pseudomonas sp.
?
L-cysteine
6035
Pseudomonas sp.
?
L-cystine
6035
Pseudomonas sp.
?
L-serine
6035
Pseudomonas sp.
?
L-serine O-sulfate
6035
Pseudomonas sp.
pyruvate + NH3 + sulfate
6035
Pseudomonas sp.
L-serine O-sulfate
6035
Pseudomonas sp. FR
pyruvate + NH3 + sulfate
6035
Pseudomonas sp. FR
L-threonine O-sulfate
6035
Pseudomonas sp.
2-oxobutanoate + NH3 + SO42-
L-threonine O-sulfate
6035
Pseudomonas sp. FR
2-oxobutanoate + NH3 + SO42-
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp.
?
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp.
?
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp. FR
?
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp. FR
?
O-acetyl-L-serine
6035
Pseudomonas sp.
?
O-benzyl DL-serine
6035
Pseudomonas sp.
?
O-benzyl-L-serine
6035
Pseudomonas sp.
?
O-diazoacetyl-L-serine
6035
Pseudomonas sp.
?
O-methyl DL-serine
6035
Pseudomonas sp.
?
O-succinyl-L-serine
6035
Pseudomonas sp.
?
S-benzyl-L-cysteine
6035
Pseudomonas sp.
?
S-carbamoyl-L-cysteine
6035
Pseudomonas sp.
?
S-ethyl-L-cysteine
6035
Pseudomonas sp.
?
S-methyl L-cysteine
6035
Pseudomonas sp.
?
S-methyl-L-cysteine
growth pattern is similar to that observed with L-serine O-sulfate
6035
Pseudomonas sp.
?
S-sulfo-L-cysteine
6035
Pseudomonas sp.
?
Temperature Stability [°C]
50
enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine
Pseudomonas sp.
Inhibitors (protein specific)
additional information
Pseudomonas sp.
KM Value [mM] (protein specific)
0.14
D-serine O-sulfate
enzyme B
Pseudomonas sp.
0.17
D-Cystine
enzyme B
Pseudomonas sp.
1.1
S-Benzyl-L-cysteine
enzyme A
Pseudomonas sp.
1.3
O-benzyl-L-serine
enzyme A
Pseudomonas sp.
2
L-cystine
enzyme A
Pseudomonas sp.
2
O-benzyl-DL-serine
enzyme B, calculated on the basis of the non-reactivity of the L-isomer
Pseudomonas sp.
2.2
D-cystathionine
enzyme B
Pseudomonas sp.
5.4
L-serine O-sulfate
enzyme B
Pseudomonas sp.
7.9
L-serine O-sulfate
enzyme A
Pseudomonas sp.
8.3
D-serine
enzyme B
Pseudomonas sp.
8.5
L-cystathionine
enzyme B
Pseudomonas sp.
9.1
L-cystathionine
enzyme A
Pseudomonas sp.
17.8
S-methyl-L-cysteine
enzyme A
Pseudomonas sp.
20
O-acetyl-L-serine
enzyme A
Pseudomonas sp.
38
S-ethyl-L-cysteine
enzyme A
Pseudomonas sp.
56
S-methyl-L-cysteine
enzyme B
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
additional information
Pseudomonas sp.
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
additional information
Pseudomonas sp. FR
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
S-methyl-L-cysteine
Pseudomonas sp.
growth pattern is similar to that observed with L-serine O-sulfate
?
Purification (Commentary) (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
additional information
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp.
?
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp. FR
?
3-cyano-L-alanine
6035
Pseudomonas sp.
?
beta-ureido-alpha-aminopropionic acid
6035
Pseudomonas sp.
?
D-cystathionine
6035
Pseudomonas sp.
?
D-cysteine
6035
Pseudomonas sp.
?
D-serine
6035
Pseudomonas sp.
?
D-serine-O-sulfate
6035
Pseudomonas sp.
pyruvate + NH3 + SO42-
DL-cysteine
6035
Pseudomonas sp.
?
DL-lanthionine
6035
Pseudomonas sp.
?
L-cystathionine + H2O
6035
Pseudomonas sp.
?
L-cysteine
6035
Pseudomonas sp.
?
L-cystine
6035
Pseudomonas sp.
?
L-serine
6035
Pseudomonas sp.
?
L-serine O-sulfate
6035
Pseudomonas sp.
pyruvate + NH3 + sulfate
6035
Pseudomonas sp.
L-serine O-sulfate
6035
Pseudomonas sp. FR
pyruvate + NH3 + sulfate
6035
Pseudomonas sp. FR
L-threonine O-sulfate
6035
Pseudomonas sp.
2-oxobutanoate + NH3 + SO42-
L-threonine O-sulfate
6035
Pseudomonas sp. FR
2-oxobutanoate + NH3 + SO42-
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp.
?
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp.
?
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp. FR
?
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp. FR
?
O-acetyl-L-serine
6035
Pseudomonas sp.
?
O-benzyl DL-serine
6035
Pseudomonas sp.
?
O-benzyl-L-serine
6035
Pseudomonas sp.
?
O-diazoacetyl-L-serine
6035
Pseudomonas sp.
?
O-methyl DL-serine
6035
Pseudomonas sp.
?
O-succinyl-L-serine
6035
Pseudomonas sp.
?
S-benzyl-L-cysteine
6035
Pseudomonas sp.
?
S-carbamoyl-L-cysteine
6035
Pseudomonas sp.
?
S-ethyl-L-cysteine
6035
Pseudomonas sp.
?
S-methyl L-cysteine
6035
Pseudomonas sp.
?
S-methyl-L-cysteine
growth pattern is similar to that observed with L-serine O-sulfate
6035
Pseudomonas sp.
?
S-sulfo-L-cysteine
6035
Pseudomonas sp.
?
Temperature Stability [°C] (protein specific)
50
enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine
Pseudomonas sp.
Other publictions for EC 4.3.1.10
6031
Murakoshi
-
L-Serine O-sulphate lyase, a n ...
Allium cepa, Allium sativum, Brassica rapa subsp. oleifera, Citrullus lanatus subsp. vulgaris, Fagopyrum esculentum, Leucaena leucocephala, Mimosa pudica, Pisum sativum, Raphanus sativus, Vigna radiata var. radiata
Chem. Pharm. Bull.
25
1829-1832
1977
-
-
-
-
-
-
7
-
-
-
-
10
-
10
-
-
-
-
-
10
-
1
22
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
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-
10
-
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-
-
-
10
-
1
22
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
6035
Tudball
Substrate specificity of the L ...
Pseudomonas sp., Pseudomonas sp. FR
Biochim. Biophys. Acta
429
616-623
1976
-
-
-
-
-
-
1
16
-
-
-
3
-
2
-
-
1
-
-
-
1
-
34
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
16
-
-
-
3
-
-
-
1
-
-
1
-
34
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
6036
Tudball
-
Factors affecting the levels o ...
Rattus norvegicus
Biochim. Biophys. Acta
338
83-91
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6032
Tudball
Studies on the inhibition of t ...
Sus scrofa
Eur. J. Biochem.
40
25-29
1973
-
-
-
-
-
-
26
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
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-
-
-
-
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-
-
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-
-
-
-
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-
26
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6033
Tudball
The enzyme degradation of L-se ...
Sus scrofa
Biochem. J.
128
41-46
1972
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6037
Tudball
The enzymic degradation of L-s ...
Sus scrofa
Biochem. J.
126
187-191
1972
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6034
Tudball
-
L-Serine O-sulphate-degrading ...
Rattus norvegicus
Methods Enzymol.
17B
361-367
1971
-
-
-
-
-
-
12
1
-
-
-
-
-
1
-
-
1
-
-
1
1
1
9
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
12
-
1
-
-
-
-
-
-
-
1
-
1
1
1
9
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
6038
Tudball
The purification and propertie ...
Sus scrofa
Biochem. J.
121
747-752
1971
-
-
-
-
-
1
-
1
-
-
4
-
-
1
-
-
1
-
-
1
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
4
-
-
-
-
1
-
1
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
6040
Tudball
Short communications. The enzy ...
Sus scrofa
Biochem. J.
120
895-897
1970
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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1
-
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-
-
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-
1
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
6039
Thomas
Studies on the enzymic degrada ...
Rattus norvegicus
Biochem. J.
105
467-472
1967
-
-
-
-
-
-
9
1
-
-
-
-
-
1
-
-
1
-
-
1
2
1
9
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
1
-
-
-
-
-
-
-
1
-
1
2
1
9
-
1
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-
-
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-
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-
-
-