show 
Substrate specificity of the L-serine O-sulphate degrading activities of Pseudomonas FR
Tudball, N.; O'Neill, J.G.; Biochim. Biophys. Acta 429, 616-623 (1976)
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
D-serine O-sulfate
enzyme B
Pseudomonas sp.
0.17
-
D-Cystine
enzyme B
Pseudomonas sp.
1.1
-
S-Benzyl-L-cysteine
enzyme A
Pseudomonas sp.
1.3
-
O-benzyl-L-serine
enzyme A
Pseudomonas sp.
2
-
L-cystine
enzyme A
Pseudomonas sp.
2
-
O-benzyl-DL-serine
enzyme B, calculated on the basis of the non-reactivity of the L-isomer
Pseudomonas sp.
2.2
-
D-cystathionine
enzyme B
Pseudomonas sp.
5.4
-
L-serine O-sulfate
enzyme B
Pseudomonas sp.
7.9
-
L-serine O-sulfate
enzyme A
Pseudomonas sp.
8.3
-
D-serine
enzyme B
Pseudomonas sp.
8.5
-
L-cystathionine
enzyme B
Pseudomonas sp.
9.1
-
L-cystathionine
enzyme A
Pseudomonas sp.
17.8
-
S-methyl-L-cysteine
enzyme A
Pseudomonas sp.
20
-
O-acetyl-L-serine
enzyme A
Pseudomonas sp.
38
-
S-ethyl-L-cysteine
enzyme A
Pseudomonas sp.
56
-
S-methyl-L-cysteine
enzyme B
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas sp.
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
-
-
-
additional information
Pseudomonas sp. FR
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
-
-
-
S-methyl-L-cysteine
Pseudomonas sp.
growth pattern is similar to that observed with L-serine O-sulfate
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
FR
-
Pseudomonas sp. FR
-
FR
-
Purification (Commentary)
Commentary
Organism
enzyme A
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp.
?
-
-
-
-
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp. FR
?
-
-
-
-
3-cyano-L-alanine
-
6035
Pseudomonas sp.
?
-
-
-
-
beta-ureido-alpha-aminopropionic acid
-
6035
Pseudomonas sp.
?
-
-
-
-
D-cystathionine
-
6035
Pseudomonas sp.
?
-
-
-
-
D-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
D-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
D-serine-O-sulfate
-
6035
Pseudomonas sp.
pyruvate + NH3 + SO42-
-
-
-
-
DL-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
DL-lanthionine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-cystathionine + H2O
-
6035
Pseudomonas sp.
?
-
-
-
-
L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-cystine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-serine O-sulfate
-
6035
Pseudomonas sp.
pyruvate + NH3 + sulfate
-
6035
Pseudomonas sp.
-
L-serine O-sulfate
-
6035
Pseudomonas sp. FR
pyruvate + NH3 + sulfate
-
6035
Pseudomonas sp. FR
-
L-threonine O-sulfate
-
6035
Pseudomonas sp.
2-oxobutanoate + NH3 + SO42-
-
-
-
-
L-threonine O-sulfate
-
6035
Pseudomonas sp. FR
2-oxobutanoate + NH3 + SO42-
-
-
-
-
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp.
?
-
-
-
-
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp.
?
-
-
-
-
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp. FR
?
-
-
-
-
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp. FR
?
-
-
-
-
O-acetyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-benzyl DL-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-benzyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-diazoacetyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-methyl DL-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-succinyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-benzyl-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-carbamoyl-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-ethyl-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-methyl L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-methyl-L-cysteine
growth pattern is similar to that observed with L-serine O-sulfate
6035
Pseudomonas sp.
?
-
-
-
-
S-sulfo-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
D-serine O-sulfate
enzyme B
Pseudomonas sp.
0.17
-
D-Cystine
enzyme B
Pseudomonas sp.
1.1
-
S-Benzyl-L-cysteine
enzyme A
Pseudomonas sp.
1.3
-
O-benzyl-L-serine
enzyme A
Pseudomonas sp.
2
-
L-cystine
enzyme A
Pseudomonas sp.
2
-
O-benzyl-DL-serine
enzyme B, calculated on the basis of the non-reactivity of the L-isomer
Pseudomonas sp.
2.2
-
D-cystathionine
enzyme B
Pseudomonas sp.
5.4
-
L-serine O-sulfate
enzyme B
Pseudomonas sp.
7.9
-
L-serine O-sulfate
enzyme A
Pseudomonas sp.
8.3
-
D-serine
enzyme B
Pseudomonas sp.
8.5
-
L-cystathionine
enzyme B
Pseudomonas sp.
9.1
-
L-cystathionine
enzyme A
Pseudomonas sp.
17.8
-
S-methyl-L-cysteine
enzyme A
Pseudomonas sp.
20
-
O-acetyl-L-serine
enzyme A
Pseudomonas sp.
38
-
S-ethyl-L-cysteine
enzyme A
Pseudomonas sp.
56
-
S-methyl-L-cysteine
enzyme B
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas sp.
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
-
-
-
additional information
Pseudomonas sp. FR
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
?
-
-
-
S-methyl-L-cysteine
Pseudomonas sp.
growth pattern is similar to that observed with L-serine O-sulfate
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
enzyme A
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp.
?
-
-
-
-
3-chloro-L-alanine methyl ester
3-chloro-L-alanine
6035
Pseudomonas sp. FR
?
-
-
-
-
3-cyano-L-alanine
-
6035
Pseudomonas sp.
?
-
-
-
-
beta-ureido-alpha-aminopropionic acid
-
6035
Pseudomonas sp.
?
-
-
-
-
D-cystathionine
-
6035
Pseudomonas sp.
?
-
-
-
-
D-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
D-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
D-serine-O-sulfate
-
6035
Pseudomonas sp.
pyruvate + NH3 + SO42-
-
-
-
-
DL-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
DL-lanthionine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-cystathionine + H2O
-
6035
Pseudomonas sp.
?
-
-
-
-
L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-cystine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
L-serine O-sulfate
-
6035
Pseudomonas sp.
pyruvate + NH3 + sulfate
-
6035
Pseudomonas sp.
-
L-serine O-sulfate
-
6035
Pseudomonas sp. FR
pyruvate + NH3 + sulfate
-
6035
Pseudomonas sp. FR
-
L-threonine O-sulfate
-
6035
Pseudomonas sp.
2-oxobutanoate + NH3 + SO42-
-
-
-
-
L-threonine O-sulfate
-
6035
Pseudomonas sp. FR
2-oxobutanoate + NH3 + SO42-
-
-
-
-
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp.
?
-
-
-
-
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp.
?
-
-
-
-
additional information
multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers
6035
Pseudomonas sp. FR
?
-
-
-
-
additional information
Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B)
6035
Pseudomonas sp. FR
?
-
-
-
-
O-acetyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-benzyl DL-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-benzyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-diazoacetyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-methyl DL-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
O-succinyl-L-serine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-benzyl-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-carbamoyl-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-ethyl-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-methyl L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
S-methyl-L-cysteine
growth pattern is similar to that observed with L-serine O-sulfate
6035
Pseudomonas sp.
?
-
-
-
-
S-sulfo-L-cysteine
-
6035
Pseudomonas sp.
?
-
-
-
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine
Pseudomonas sp.
Other publictions for EC 4.3.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
6031
Murakoshi
-
L-Serine O-sulphate lyase, a n ...
Allium cepa, Allium sativum, Brassica rapa subsp. oleifera, Citrullus lanatus subsp. vulgaris, Fagopyrum esculentum, Leucaena leucocephala, Mimosa pudica, Pisum sativum, Raphanus sativus, Vigna radiata var. radiata
Chem. Pharm. Bull.
25
1829-1832
1977
-
-
-
-
-
-
7
-
-
-
-
10
-
10
-
-
-
-
-
10
-
1
22
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
10
-
-
-
-
-
10
-
1
22
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
6035
Tudball
Substrate specificity of the L ...
Pseudomonas sp., Pseudomonas sp. FR
Biochim. Biophys. Acta
429
616-623
1976
-
-
-
-
-
-
1
16
-
-
-
3
-
2
-
-
1
-
-
-
1
-
34
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
16
-
-
-
3
-
-
-
1
-
-
1
-
34
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
6036
Tudball
-
Factors affecting the levels o ...
Rattus norvegicus
Biochim. Biophys. Acta
338
83-91
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6032
Tudball
Studies on the inhibition of t ...
Sus scrofa
Eur. J. Biochem.
40
25-29
1973
-
-
-
-
-
-
26
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
26
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6033
Tudball
The enzyme degradation of L-se ...
Sus scrofa
Biochem. J.
128
41-46
1972
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6037
Tudball
The enzymic degradation of L-s ...
Sus scrofa
Biochem. J.
126
187-191
1972
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6034
Tudball
-
L-Serine O-sulphate-degrading ...
Rattus norvegicus
Methods Enzymol.
17B
361-367
1971
-
-
-
-
-
-
12
1
-
-
-
-
-
1
-
-
1
-
-
1
1
1
9
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
12
-
1
-
-
-
-
-
-
-
1
-
1
1
1
9
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
6038
Tudball
The purification and propertie ...
Sus scrofa
Biochem. J.
121
747-752
1971
-
-
-
-
-
1
-
1
-
-
4
-
-
1
-
-
1
-
-
1
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
4
-
-
-
-
1
-
1
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
6040
Tudball
Short communications. The enzy ...
Sus scrofa
Biochem. J.
120
895-897
1970
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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6039
Thomas
Studies on the enzymic degrada ...
Rattus norvegicus
Biochem. J.
105
467-472
1967
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