Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Escherichia coli |
30400 | - |
calculated from amino acid sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli KSR7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography, HiTrap heparin column chromatography, HiTrap Blue column chromatography, glutathione-Sepharose 4B column chromatography, hydroxyapatite column chromatography, and Resource S column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | KsgA has al DNA glycosylase/AP lyase activity for C mispaired with oxidized T | Escherichia coli | fragments of DNA | - |
? | |
DNA | KsgA has al DNA glycosylase/AP lyase activity for C mispaired with oxidized T | Escherichia coli KSR7 | fragments of DNA | - |
? | |
additional information | KsgA does not remove C opposite normal bases, 7,8-dihydro-8-oxoguanine and 2-hydroxyadenine, KsgA does not excise thymine glycol, 5-formyluracil, and 5-hydroxymethyluracil opposite cytosine from double-stranded oligonucleotides | Escherichia coli | ? | - |
? | |
additional information | KsgA does not remove C opposite normal bases, 7,8-dihydro-8-oxoguanine and 2-hydroxyadenine, KsgA does not excise thymine glycol, 5-formyluracil, and 5-hydroxymethyluracil opposite cytosine from double-stranded oligonucleotides | Escherichia coli KSR7 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KsgA | a 16S rRNA adenine methyltransferase with DNA glycosylase/AP lyase activity | Escherichia coli |