Cloned (Comment) | Organism |
---|---|
recombinant aristolochene synthase is expressed in Escherichia coli BL21(DE3)pLysS | Aspergillus terreus |
Crystallization (Comment) | Organism |
---|---|
crystallization by the hanging drop, vapor diffusion method at 4°C, the crystal structure determined from crystals soaked with farnesyl diphosphate reveals the binding of intact farnesyl diphosphate to monomers A-C, and the binding of diphosphate anion and Mg2+ to monomer D. The structure of the complex with 2-fluorofarnesyl diphosphate reveals 2-fluorofarnesyl diphosphate binding to all subunits of the tetramer, with Mg2+B accompanying the binding of this analogue only in monomer D. The structure of the complex with 12,13-difluorofarnesyl diphosphate reveals the binding of intact 12,13-difluorofarnesyl diphosphate to monomers A-C in the open conformation and the binding of diphosphate anion, Mg2+B, and Mg2+C to monomer D in a predominantly closed conformation | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
12,13-difluorofarnesyl diphosphate | incubation of 12,13-difluorofarnesyl diphosphate with for 30 h does not generate any pentane-extractable products based on GC-MS analysis | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme utilizes a trinuclear magnesium cluster to trigger the departure of the diphosphate leaving group, thereby forming an allylic carbocation that typically reacts with one of the remaining sigma-bonds of the substrate | Aspergillus terreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate | Aspergillus terreus | The universal sesquiterpene precursor farnesyl diphosphate (15-carbon isoprenoid) is cyclized in an Mg2-dependent reaction to form the bicyclic hydrocarbon aristolochene and a diphosphate anion coproduct | (+)-aristolochene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | Q9UR08 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-fluorofarnesyl diphosphate | two products are identified in a 95/5 ratio by GS-MS | Aspergillus terreus | 2-fluorogermacrene A + diphosphate | - |
? | |
farnesyl diphosphate | The universal sesquiterpene precursor farnesyl diphosphate (15-carbon isoprenoid) is cyclized in an Mg2-dependent reaction to form the bicyclic hydrocarbon aristolochene and a diphosphate anion coproduct | Aspergillus terreus | (+)-aristolochene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution | Aspergillus terreus |
tetramer | crystal structure | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
aristolochene synthase | sesquiterpene cyclase | Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Aspergillus terreus |