Cloned (Comment) | Organism |
---|---|
functional expression of Picea abies isopimara-7,15-diene synthase (diTPS-ISO) in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection method, coexpression with a multifunctional cytochrome P450 (CYP720B4) from Picea sitchensis, subcloning in Escherichia coli strain NEB10beta | Picea abies |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transient heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana. Expressing DXS and GGPPs from Plectranthus barbatus in Nicotiana benthamiana are efficient in boosting diterpenoid levels compared to coexpression of the Nicotiana endogenous DXS and GGPPs genes, which have no effect on boosting diterpenoid level. Pigment analysis of infiltrated leaves, overview | Picea abies |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Picea abies | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Picea abies |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | Picea abies | - |
isopimara-7,15-diene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | Q675L5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Picea abies | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | - |
Picea abies | isopimara-7,15-diene + diphosphate | - |
? | |
additional information | the bifunctional diterpene synthase isopimara-7,15-diene synthase (diTPS-ISO) catalyzes the cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate (cf. EC 5.5.1.12) and the formation of isopimara-7,15-diene from (+)-copalyl diphosphate. Compound identification and quantification by GC-MS analysis | Picea abies | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diTPS-ISO | - |
Picea abies |
isopimara-7, 15-diene synthase | - |
Picea abies |
More | cf. EC 5.5.1.12 | Picea abies |
General Information | Comment | Organism |
---|---|---|
metabolism | the bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) and a multifunctional cytochrome P450, CYP720B4 perform the biosynthesis of isopimaric acid. The diterpene synthase converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene) via formation of (+)-copalyl diphosphate. In three subsequent oxygenation reactions, the endoplasmic reticulum-associated enzyme CYP720B4 catalyzes the conversion of isopimaradiene into isopimaric acid, pathway overview | Picea abies |
physiological function | the chloroplastidic bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) from Picea abies and a cytosolic multifunctional cytochrome P450 (CYP720B4, UniProt ID E5FA70) from Picea sitchensis, which is associated to the endoplasmic reticulum. The diterpene synthase diTPS-ISO also converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene) | Picea abies |