Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 10% (w/v) PEG 4000 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
Q62R | inactive | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Crh protein | in the absence of preferred carbon sources, the phosphoprotein Crh is present in the nonphosphorylated state and binds to and thereby inhibits the enzyme | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate | Bacillus subtilis | - |
2-oxopropanal + phosphate | - |
? | |
glycerone phosphate | Bacillus subtilis 168 | - |
2-oxopropanal + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P42980 | - |
- |
Bacillus subtilis 168 | P42980 | - |
- |
Purification (Comment) | Organism |
---|---|
Strep-Tactin column chromatography and Sepharose S75 gel filtration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
wild type enzyme, at pH 7.5 and 30°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate | - |
Bacillus subtilis | 2-oxopropanal + phosphate | - |
? | |
glycerone phosphate | - |
Bacillus subtilis 168 | 2-oxopropanal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
MgsA | - |
Bacillus subtilis |