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Literature summary for 4.2.3.27 extracted from

  • Lehning, A.; Zimmer, I.; Steinbrecher, R.; Bruggemann, N.; Schnitzler, J.P.
    Isoprene synthase activity and its relation to isoprene emission in Quercus robur L. leaves (1999), Plant Cell Environ., 22, 495-504.
No PubMed abstract available
Search for more literature for 4.2.3.27

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme activity is induced by light, positive regulation Quercus robur

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Quercus robur

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Quercus robur the enzyme is positively regulated by light isoprene + diphosphate isoprene is emitted into the atmosphere following a diurnal cycle in vivo ir

Organism

Organism UniProt Comment Textmining
Quercus robur
-
 penduculate oak
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from leaves by ammonium sulfate fractionation Quercus robur

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Quercus robur
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Quercus robur is a strong emitter of isoprene, measurements of three trees over three days Quercus robur
0.333
-
 partially purified enzyme Quercus robur

Storage Stability

Storage Stability Organism
-80°C, 30-60°C ammonium sulfate fraction, resuspended in 50 mM Tris-HCl, pH 8.5, 20 mM MgCl2, 5% glycerol, 2 mM DTT, stable Quercus robur

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate
-
 Quercus robur isoprene + diphosphate i.e. 2-methyl-1,3-butadiene, product determination by gas chromatography ir
dimethylallyl diphosphate the enzyme is positively regulated by light Quercus robur isoprene + diphosphate isoprene is emitted into the atmosphere following a diurnal cycle in vivo ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Quercus robur

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 exponential increase of activity from 15°C to 40°C, optimum activity at 50°C, inactivation at 60°C Quercus robur

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 first optimum Quercus robur
7.7
-
 second optimum Quercus robur

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9.2 sharp decrease in activity below pH 6.3 Quercus robur