Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli | Cichorium intybus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Cichorium intybus | |
0.0032 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 30°C | Cichorium intybus | |
0.0069 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 30°C | Cichorium intybus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | Cichorium intybus | - |
(+)-(10R)-germacrene A + diphosphate | - |
? | |
additional information | Cichorium intybus | (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cichorium intybus | Q8LSC2 | short enzyme form, mRNA; 2 isozymes encoded by 2 different genes | - |
Cichorium intybus | Q8LSC3 | long enzyme form, mRNA; 2 isozymes encoded by 2 different genes | - |
Purification (Comment) | Organism |
---|---|
recombinant long isozyme from Escherichia coli, native isozyme partially from chicory heads by anion exchange chromatography | Cichorium intybus |
recombinant short isozyme from Escherichia coli, native isozyme partially from chicory heads by anion exchange chromatography | Cichorium intybus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate = (+)-(R)-gemacrene A + diphosphate | structure modeling and reaction mechanism, overview | Cichorium intybus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | head leaves, very low expression in green leaves | Cichorium intybus | - |
additional information | both isozymes are expressed in all tissues but in varying levels, overview | Cichorium intybus | - |
additional information | nearly no expression of the short isozyme in head leaves and in green leaves, both isozymes are expressed in all tissues but in varying levels, overview | Cichorium intybus | - |
seedling | normal and etiolated | Cichorium intybus | - |
shoot | head core tissue | Cichorium intybus | - |
taproot | especially in outer tissue, highest expression level of the long isozyme | Cichorium intybus | - |
taproot | highest expression level of the short isozyme in outer and inner tissues | Cichorium intybus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Cichorium intybus | (+)-(10R)-germacrene A + diphosphate | - |
? | |
additional information | (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory | Cichorium intybus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isozyme primary sequence and three-dimensional structure modeling | Cichorium intybus |
Synonyms | Comment | Organism |
---|---|---|
GASlo | - |
Cichorium intybus |
GASsh | - |
Cichorium intybus |
germacrene A synthase | - |
Cichorium intybus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Cichorium intybus |
7 | - |
- |
Cichorium intybus |