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Literature summary for 4.2.3.19 extracted from

  • Zerbe, P.; Chiang, A.; Bohlmann, J.
    Mutational analysis of white spruce (Picea glauca) ent-kaurene synthase (PgKS) reveals common and distinct mechanisms of conifer diterpene synthases of general and specialized metabolism (2012), Phytochemistry, 74, 30-39.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3-C41 cells Picea glauca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Picea glauca ent-copalyl diphosphate is converted in an ionization-initiated (class I) reaction that involves formation of a pimarenyl carbocation intermediate, followed by secondary cyclization to a beyeranyl cation which undergoes ring rearrangement and deprotonation to form the tetracyclic ent-kaurene ent-kaurene + diphosphate
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Organism

Organism UniProt Comment Textmining
Picea glauca
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Purification (Commentary)

Purification (Comment) Organism
HisSpinTrap column chromatography Picea glauca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate ent-copalyl diphosphate is converted in an ionization-initiated (class I) reaction that involves formation of a pimarenyl carbocation intermediate, followed by secondary cyclization to a beyeranyl cation which undergoes ring rearrangement and deprotonation to form the tetracyclic ent-kaurene Picea glauca ent-kaurene + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
class I diTPS ent-kaurene synthase
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 Picea glauca