BRENDA - Enzyme Database show
show all sequences of 4.2.3.150

Two diterpene synthases for spiroalbatene and cembrene A from allokutzneria albata

Rinkel, J.; Lauterbach, L.; Rabe, P.; Dickschat, J.; Angew. Chem. Int. Ed. Engl. 57, 3238-3241 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
phylogenetic tree, recombinant expression of wild-type and mutant enzymes in Escherichia coli
Allokutzneria albata
Engineering
Amino acid exchange
Commentary
Organism
D212E
site-directed mutagenesis, the mutation results in strongly decreased activity with Mg2+ and Mn2+
Allokutzneria albata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activates
Allokutzneria albata
Mn2+
activates
Allokutzneria albata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geranylgeranyl diphosphate
Allokutzneria albata
-
(S)-(+)-cembrene A + diphosphate
-
-
?
geranylgeranyl diphosphate
Allokutzneria albata DSM 44149
-
(S)-(+)-cembrene A + diphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Allokutzneria albata
-
isolated from tropical soil
-
Allokutzneria albata DSM 44149
-
isolated from tropical soil
-
Reaction
Reaction
Commentary
Organism
geranylgeranyl diphosphate = (R)-cembrene A + diphosphate
the cyclization mechanism from geranylgeranyl diphosphate to cembrene A requires a 1,14-cyclization to the ent-cembranyl cation and deprotonation of one of the geminal methyl groups, reaction mechanism, overview
Allokutzneria albata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme seems to be not expressed in laboratory cultures
Allokutzneria albata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geranylgeranyl diphosphate
-
746752
Allokutzneria albata
(S)-(+)-cembrene A + diphosphate
-
-
-
?
geranylgeranyl diphosphate
-
746752
Allokutzneria albata DSM 44149
(S)-(+)-cembrene A + diphosphate
-
-
-
?
additional information
product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes
746752
Allokutzneria albata
?
-
-
-
-
additional information
product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes
746752
Allokutzneria albata DSM 44149
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
phylogenetic tree, recombinant expression of wild-type and mutant enzymes in Escherichia coli
Allokutzneria albata
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D212E
site-directed mutagenesis, the mutation results in strongly decreased activity with Mg2+ and Mn2+
Allokutzneria albata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activates
Allokutzneria albata
Mn2+
activates
Allokutzneria albata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geranylgeranyl diphosphate
Allokutzneria albata
-
(S)-(+)-cembrene A + diphosphate
-
-
?
geranylgeranyl diphosphate
Allokutzneria albata DSM 44149
-
(S)-(+)-cembrene A + diphosphate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme seems to be not expressed in laboratory cultures
Allokutzneria albata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geranylgeranyl diphosphate
-
746752
Allokutzneria albata
(S)-(+)-cembrene A + diphosphate
-
-
-
?
geranylgeranyl diphosphate
-
746752
Allokutzneria albata DSM 44149
(S)-(+)-cembrene A + diphosphate
-
-
-
?
additional information
product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes
746752
Allokutzneria albata
?
-
-
-
-
additional information
product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes
746752
Allokutzneria albata DSM 44149
?
-
-
-
-
Other publictions for EC 4.2.3.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746752
Rinkel
Two diterpene synthases for s ...
Allokutzneria albata, Allokutzneria albata DSM 44149
Angew. Chem. Int. Ed. Engl.
57
3238-3241
2018
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1
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1
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2
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2
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2
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1
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1
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4
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1
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1
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2
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2
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1
-
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4
-
-
-
-
-
-
-
-
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747151
Ansbacher
Slow-starter enzymes role of ...
Taxus brevifolia
Biochemistry
57
3773-3779
2018
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-
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2
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-
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-
-
1
-
-
-
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1
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1
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746593
Tomita
Structural insights into the ...
Streptomyces melanosporofaciens, Streptomyces melanosporofaciens MI614-43F2
ACS Chem. Biol.
12
1621-1628
2017
-
-
1
-
3
-
-
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1
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2
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3
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1
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6
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1
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1
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1
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3
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1
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6
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1
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1
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729596
Meguro
Identification and characteriz ...
Streptomyces sp., Streptomyces sp. SANK 60404
ChemBioChem
14
316-321
2013
-
-
1
-
-
-
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1
-
1
2
2
-
2
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1
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4
1
1
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1
1
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1
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1
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1
2
2
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1
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4
1
1
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1
1
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