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Literature summary for 4.2.3.141 extracted from

  • Pan, X.; Han, L.; Zhang, Y.; Chen, D.; Simonsen, H.
    Sclareol production in the moss Physcomitrella patens and observations on growth and terpenoid biosynthesis (2015), Plant Biotechnol. Rep., 9, 149-159 .
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Cloned(Commentary)

Cloned (Comment) Organism
coexpression of gene SsLPPS encoding labd-13-en-8-ol diphosphate synthase (EC 4.2.1.133) and gene SsSS encoding sclareol synthase, recombinant enzyme expression in Physcomitrella patens from vectors pUNI33-SsLPPS-2A-SsSS and pBK3-SsLPPS-2A-SsSS, RT-PCR enzyme expression analysis, overview Salvia sclarea

Protein Variants

Protein Variants Comment Organism
additional information coexpression of gene SsLPPS encoding labd-13-en-8-ol diphosphate synthase (EC 4.2.1.133) and gene SsSS encoding sclareol synthase, recombinant enzyme expression in Physcomitrella patens from vectors pUNI33-SsLPPS-2A-SsSS and pBK3-SsLPPS-2A-SsSS. The transcript levels of SsSS and SsLPPS cannot be directly correlated to the final yields for sclareol of the different transgenic lines, but it is observed that lines with high transcript levels of the genes also have high sclareol levels. The highest yield is 2.84 lg/mg dry weight (2.28 lg/ml) in a liquid culture of wild-type line 2 (WTF2), including sclareol found in the media and in the moss cells. Enzyme expression analysis, overview. Transcriptional changes in the 14-day-old WTF2 shows a similar down-regulation for most genes, but not for HDR, IDI, and HMGS. The HDR, IDI, and HMGS show a higher expression level than in those in the wild-type. Besides sclareol, the three endogenous ent-kaurenoids (ent-kaur-15-ene, ent-kaur-16-ene and ent-16-hydroxy- kaurene) are also observed in the WTF2, but the peaks are significantly lower than in the wild-type line. GC-MS ligand analysis. Of all transgenic lines, the WTF2 line has the most growth impairment and stops growing after 15 days. general, general growth deficiency for transformed lines Salvia sclarea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O Salvia sclarea
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 sclareol + diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Salvia sclarea K4HYB0
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O
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 Salvia sclarea sclareol + diphosphate
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 ?
additional information GC-MS reaction product analysis Salvia sclarea ?
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 ?

Synonyms

Synonyms Comment Organism
SsSS
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 Salvia sclarea