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Literature summary for 4.2.3.103 extracted from
- Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon (2016), Biochem. Biophys. Res. Commun., 480, 402-408 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene OrKSL5ind, sequence comparisons and phylogenetic analysis and tree | Oryza rufipogon |
| gene OrKSL6, sequence comparisons and phylogenetic analysis and tree | Oryza rufipogon |
| gene OrKSL6ind, sequence comparisons and phylogenetic analysis and tree | Oryza rufipogon |
| gene OsKSL5i, sequence comparisons and phylogenetic analysis and tree | Oryza sativa Indica Group |
| gene OsKSL6, sequence comparisons and phylogenetic analysis and tree | Oryza sativa Japonica Group |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Oryza rufipogon | 9507 | - |
| chloroplast | - |
Oryza sativa Japonica Group | 9507 | - |
| chloroplast | - |
Oryza sativa Indica Group | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Oryza rufipogon |  |
| Mg2+ | required | Oryza sativa Japonica Group | |
| Mg2+ | required | Oryza sativa Indica Group | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ent-copalyl diphosphate | Oryza rufipogon | - |
ent-isokaurene + diphosphate | - |
? | |
| ent-copalyl diphosphate | Oryza sativa Japonica Group | - |
ent-isokaurene + diphosphate | - |
? | |
| ent-copalyl diphosphate | Oryza sativa Indica Group | - |
ent-isokaurene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza rufipogon | - |
- |
- |
| Oryza rufipogon | LC169118 | - |
- |
| Oryza sativa Indica Group | A4KAG7 | - |
- |
| Oryza sativa Japonica Group | A4KAG8 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza rufipogon | - |
| leaf | - |
Oryza sativa Japonica Group | - |
| leaf | - |
Oryza sativa Indica Group | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ent-copalyl diphosphate | - |
Oryza rufipogon | ent-isokaurene + diphosphate | - |
? | |
| ent-copalyl diphosphate | - |
Oryza sativa Japonica Group | ent-isokaurene + diphosphate | - |
? | |
| ent-copalyl diphosphate | - |
Oryza sativa Indica Group | ent-isokaurene + diphosphate | - |
? | |
| additional information | recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene | Oryza rufipogon | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ent-kaurene synthase like | - |
Oryza rufipogon |
| ent-kaurene synthase like | - |
Oryza sativa Indica Group |
| ent-kaurene synthase like 5 | - |
Oryza rufipogon |
| ent-kaurene synthase like 6 | - |
Oryza sativa Japonica Group |
| KSL5i | - |
Oryza sativa Indica Group |
| KSL5ind | - |
Oryza rufipogon |
| KSL6 | - |
Oryza sativa Japonica Group |
| KSL6 | - |
Oryza rufipogon |
| KSL6ind | - |
Oryza rufipogon |
| OrKSL5ind | - |
Oryza rufipogon |
| OrKSL6 | - |
Oryza rufipogon |
| OrKSL6ind | - |
Oryza rufipogon |
| OsKSL5i | - |
Oryza sativa Indica Group |
| OsKSL6 | - |
Oryza sativa Japonica Group |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzymes KSLs belong to the type A/class I terpene synthase family. The KSL5 members are clearly divided into two clades, representing japonica and indica groups. The KS responsible for gibberellin biosynthesis in a putative common ancestor, and OsKS1, which produces the tetracyclic ent-kaurene, has Ile as the corresponding key amino acid. Thus, it is suggested that substitution from Ile664 to Thr664 in KSL5 occurred before the onset of cultivation in the putative japonica ancestor. The OsKSL5 sequence is similar to the OsKSL6 sequence (89% identity), and it is therefore likely that the two KSL genes were generated through relatively recent duplication. The substitution in KSL5 probably occurred after duplication of the ancestral gene | Oryza sativa Indica Group |
| physiological function | OsKSL5i and OsKSL6, encoding ent-isokaurene synthase, are not responsible for phytocassane biosynthesis, but are suggested to be involved in other specialized diterpenoid metabolism including the synthesis of oryzadiones | Oryza sativa Japonica Group |
| physiological function | OsKSL5i and OsKSL6, encoding ent-isokaurene synthase, are not responsible for phytocassane biosynthesis, but are suggested to be involved in other specialized diterpenoid metabolism including the synthesis of oryzadiones. The KSL5 members are clearly divided into two clades, representing japonica and indica groups, the 664th amino acids of OsKSL5j and OsKSL5i are Thr and Ile leading to different activity and production of ent-isokeurene synthase (EC 4.2.3.103) or ent-pimara-8(14),15-diene (EC 4.2.3.30) | Oryza sativa Indica Group |
| physiological function | OsKSL5ind and OsKSL6, encoding ent-isokaurene synthase, are not responsible for phytocassane biosynthesis, but are suggested to be involved in other specialized diterpenoid metabolism including the synthesis of oryzadiones. Recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene. The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively | Oryza rufipogon |
| physiological function | recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene | Oryza rufipogon |
| physiological function | recombinant OrKSL5 converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene (cf. EC 4.2.3.30), whereas OrKSL5ind, OrKSL6 and OrKSL6ind all convert ent-copalyl diphosphate to ent-isokaurene. The 664th amino acids of OrKSL5 and OrKSL5ind are Thr and Ile, respectively | Oryza rufipogon |
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