Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 72% residual activity | Microbulbifer sp. ALW1 | |
Ca2+ | 1 mM, 6% residual activity | Microbulbifer sp. ALW1 | |
Co2+ | 1 mM, 11% residual activity | Microbulbifer sp. ALW1 | |
Cu2+ | 1 mM, 10% residual activity | Microbulbifer sp. ALW1 | |
EDTA | 1 mM, 50% residual activity | Microbulbifer sp. ALW1 | |
Fe3+ | 1 mM, 11% residual activity | Microbulbifer sp. ALW1 | |
Ni2+ | 1 mM, 42% residual activity | Microbulbifer sp. ALW1 | |
Zn2+ | 1 mM, 32% residual activity | Microbulbifer sp. ALW1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
alginate | Km value 1.03 ml/mg/s, pH 7.0, 45°C | Microbulbifer sp. ALW1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Microbulbifer sp. ALW1 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | 1 mM, 115% of initial activity | Microbulbifer sp. ALW1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Microbulbifer sp. ALW1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbulbifer sp. ALW1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification by centrifugation, ammonium sulfate fractionation, anion-exchange chromatography and size exclusion chromatography | Microbulbifer sp. ALW1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Microbulbifer sp. ALW1 | unsaturated algino-oligosaccharides | - |
? | |
poly(alpha-1,4-L-guluronate) | reaction of EC 4.2.2.11 | Microbulbifer sp. ALW1 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
poly(beta-1,4-D-mannuronate) | about 50% of the activity with poly(alpha-1,4-L-guluronate) or alginate | Microbulbifer sp. ALW1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26000, SDS-PAGE | Microbulbifer sp. ALW1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Microbulbifer sp. ALW1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | at least 35% of maximum activity | Microbulbifer sp. ALW1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
1 h, 68% residual activity | Microbulbifer sp. ALW1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69.4 | - |
alginate | pH 7.0, 45°C | Microbulbifer sp. ALW1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Microbulbifer sp. ALW1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | at least 30% of maximum activity | Microbulbifer sp. ALW1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
alginate | kcat/Km value 67.4 mg/ml, pH 7.0, 45°C | Microbulbifer sp. ALW1 |