Application | Comment | Organism |
---|---|---|
medicine | it might be possible to use alginate lyase AlgL as an adjuvant therapeutic medicine for the treatment of disease associated with Pseudomonas aeruginosa infection | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
algL is expressed in pET24a (+)/E. coli BL21 (DE3) system | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 18% inhibition | Pseudomonas sp. | |
Fe3+ | 1 mM, complete inhibition | Pseudomonas sp. | |
Ni2+ | 1 mM, 27% inhibition | Pseudomonas sp. | |
SDS | 1 mM, complete inhibition | Pseudomonas sp. | |
Zn2+ | 1 mM, 21% inhibition | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 4.2fold increase in activity | Pseudomonas sp. | |
Ca2+ | 1 mM, 2.5fold increase in activity | Pseudomonas sp. | |
K+ | 1 mM, 4.2fold increase in activity | Pseudomonas sp. | |
Mg2+ | 1 mM, 3.1fold increase in activity | Pseudomonas sp. | |
Mn2+ | 1 mM, 3.2fold increase in activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42200 | - |
x * 42200, calculated from sequence | Pseudomonas sp. |
42800 | - |
x * 42800, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q597B8 | - |
- |
Pseudomonas sp. QD03 | Q597B8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | can degrade alginate and mannuronate blocks, but hardly degrades guluronate blocks. In particular, AlgL can degrade acetylated alginate of Pseudomonas aeruginosa FRD1 | Pseudomonas sp. | ? | - |
? | |
alginate | can degrade alginate and mannuronate blocks, but hardly degrades guluronate blocks. In particular, AlgL can degrade acetylated alginate of Pseudomonas aeruginosa FRD1 | Pseudomonas sp. QD03 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42800, SDS-PAGE | Pseudomonas sp. |
? | x * 42200, calculated from sequence | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
AlgL | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 25°C: about 55% of maximal activity, 45°C: about 65% of maximal activity | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, about 10% loss of activity | Pseudomonas sp. |
40 | - |
1 h, about 20% loss of activity | Pseudomonas sp. |
50 | - |
1 h, about 40% loss of activity | Pseudomonas sp. |
60 | - |
1 h, about 75% loss of activity | Pseudomonas sp. |
80 | - |
1 h, complete loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 45% of maximal activity, pH 8.0: about 50% of maximal activity | Pseudomonas sp. |