Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 50% inhibition | unidentified microorganism | |
Hg2+ | 0.1 mM, significantly inhibits | unidentified microorganism | |
additional information | Ca2+, Mo2+, Cu2+, Fe2+, Co2+, and Zn2+ show no effects on enzyme activity at 1 mM. Dithiothreitol, glutathione (reduced form), and 2-mercaptoethanol (1 mM each) and N-ethylmaleimide (0.1 mM) exhibit no effect on enzyme activity | unidentified microorganism |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
1 * 140000, SDS-PAGE | unidentified microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified microorganism | - |
soil microorganism, microbial strain for lyase production not specified | - |
Purification (Comment) | Organism |
---|---|
microbial strain for lyase production not specified | unidentified microorganism |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
unidentified microorganism | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.671 | - |
pH 7.0, 30°C | unidentified microorganism |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | the enzyme is highly specific to gellan, especially deacetylated gellan, and other gellan-related polysaccharides, such as S-88, welan, rhamsan, and S-198. The viscosity of the gellan solution in the presence of the enzyme decreases and finally reaches a level comparable to that of water | unidentified microorganism | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 140000, SDS-PAGE | unidentified microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
unidentified microorganism |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 45% of maximal activity, 50°C: 50% of maximal activity | unidentified microorganism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 7.5, stable below | unidentified microorganism |
50 | - |
pH 7.5, 10 min, 50% loss of activity | unidentified microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in sodium N-2-hydroxyethylpiperazine-N'-2-ethanesulfonate (HEPES) buffer | unidentified microorganism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity | unidentified microorganism |
Organism | Comment | Expression |
---|---|---|
unidentified microorganism | indicible in presence of gellan | up |