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Literature summary for 4.2.2.23 extracted from

  • Dhillon, A.; Fernandes, V.O.; Dias, F.M.; Prates, J.A.; Ferreira, L.M.; Fontes, C.M.; Centeno, M.S.; Goyal, A.
    A New member of family 11 polysaccharide lyase, rhamnogalacturonan lyase (CtRGLf) from Clostridium thermocellum (2016), Mol. Biotechnol., 58, 232-240 .
    View publication on PubMed
Search for more literature for 4.2.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
additional information expression of a protein derivative without the N-terminal Dockerin I. Truncated protein has a molecular mass of 63900 Da Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 pectic galactan Km value of wild-type 10 mg/ml, of truncated mutant 11.2 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 potato galactan Km value of wild-type 4.7 mg/ml, of truncated mutant 6.4 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 soybean rhamnogalacturonan I Km value of wild-type 4.8 mg/ml, of truncated mutant 5.1 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 potato rhamnogalacturonan I Km value of wild-type 4.9 mg/ml, of truncated mutant 6.0 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 Pectin Km value of wild-type 8.2 mg/ml, of truncated mutant 7.6 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 3 mM, 1.5fold activation of wild-type, 1.3fold activation of truncated mutant protein of 63900 Da Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DC06
-
-
Acetivibrio thermocellus DSM 1237 A3DC06
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 substrate soybean rhamnogalacturonan I, pH 8.5, temperature not specified in the publication Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectic galactan
-
 Acetivibrio thermocellus ?
-
 ?
pectic galactan
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
pectin 25% methyl-esterified Acetivibrio thermocellus ?
-
 ?
pectin 25% methyl-esterified Acetivibrio thermocellus DSM 1237 ?
-
 ?
potato galactan
-
 Acetivibrio thermocellus ?
-
 ?
potato galactan
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
potato rhamnogalacturonan I
-
 Acetivibrio thermocellus rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?
potato rhamnogalacturonan I
-
 Acetivibrio thermocellus DSM 1237 rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?
soybean rhamnogalacturonan I
-
 Acetivibrio thermocellus rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?
soybean rhamnogalacturonan I
-
 Acetivibrio thermocellus DSM 1237 rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?

Synonyms

Synonyms Comment Organism
RglF
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 truncated mutant protein of 63900 Da Acetivibrio thermocellus
70
-
 wild-type Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 potato galactan truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
2.3
-
 Pectin truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
4.8
-
 pectic galactan truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
6
-
 potato rhamnogalacturonan I truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
6.4
-
 potato galactan wild-type, pH 8.5, 70°C Acetivibrio thermocellus
6.9
-
 soybean rhamnogalacturonan I truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
7.7
-
 pectic galactan wild-type, pH 8.5, 70°C Acetivibrio thermocellus
9.8
-
 Pectin wild-type, pH 8.5, 70°C Acetivibrio thermocellus
21
-
 potato rhamnogalacturonan I wild-type, pH 8.5, 70°C Acetivibrio thermocellus
21.2
-
 soybean rhamnogalacturonan I wild-type, pH 8.5, 70°C Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Acetivibrio thermocellus

General Information

General Information Comment Organism
physiological function both the full-length Rgl protein (80000 Da) and its truncated derivative (63900 Da) display maximum activity against rhamnogalacturonan I Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 pectic galactan kcat/Km value of wild-type 0.77 ml/mg/s, of truncated mutant 0.43 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 Pectin kcat/Km value of wild-type 1.19 ml/mg/s, of truncated mutant 0.3 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 potato galactan kcat/Km value of wild-type 1.36 ml/mg/s, of truncated mutant 0.32 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 soybean rhamnogalacturonan I kcat/Km value of wild-type 4.1 ml/mg/s, of truncated mutant 1.35 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
additional information
-
 potato rhamnogalacturonan I kcat/Km value of wild-type 4.28 ml/mg/s, of truncated mutant 1.13 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus