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Literature summary for 4.2.2.2 extracted from
- Expression and thermostability of Paenibacillus campinasensis BL11 pectate lyase and its applications in bast fibre processing (2011), Ann. Appl. Biol., 158, 218-225.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | enzyme treatment in fibre degumming validated by weight loss in gampi and paper mulberry fibres | Paenibacillus campinasensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged Pel-BL11 in Escherichia coli pET25b | Paenibacillus campinasensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus campinasensis | D8WN00 | - |
- |
| Paenibacillus campinasensis BL11 | D8WN00 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Pel-BL11 from Escherichia coli by nickel affinity and anion exchange chromatography | Paenibacillus campinasensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1623 | - |
purified recombinant His-tagged Pel-BL11, pH 10.0, 50°C | Paenibacillus campinasensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectate | i.e. polygalacturonic acid, cleavage of the pectate alpha-1,4-glycosidic bond by Pel-BL11 | Paenibacillus campinasensis | ? | - |
? |  |
| pectate | i.e. polygalacturonic acid, cleavage of the pectate alpha-1,4-glycosidic bond by Pel-BL11 | Paenibacillus campinasensis BL11 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34500-38200, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus campinasensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pel-BL11 | - |
Paenibacillus campinasensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Paenibacillus campinasensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | activity range | Paenibacillus campinasensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, half-life is 288 min at pH 10.0 | Paenibacillus campinasensis |
| 50 | - |
purified recombinant His-tagged enzyme, stable up to for 3 h, half-lives at pH 7.0-11.0 are 327-103 min, half-life of 147 min at pH 10.0, overview | Paenibacillus campinasensis |
| 60 | - |
purified recombinant His-tagged enzyme, half-life is 207 min at pH 10.0 | Paenibacillus campinasensis |
| 70 | - |
purified recombinant His-tagged enzyme, half-life is 120 min at pH 10.0 | Paenibacillus campinasensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Paenibacillus campinasensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | activity range | Paenibacillus campinasensis |
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Release 2023.1 (January 2023)
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