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Literature summary for 4.2.2.2 extracted from
- Purification and characterization of thermostable pectate-lyases from a newly isolated thermophilic bacterium, Thermoanaerobacter italicus sp. nov (1997), Extremophiles, 1, 171-182.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Thermoanaerobacter italicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for citrus pectin: 5.0 g/l | Thermoanaerobacter italicus | |
| additional information | - |
additional information | Km for pectate: 0.5 g/l | Thermoanaerobacter italicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Thermoanaerobacter italicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
pectate lyase b shows two bands in non-denaturing PAGE: 200000 Da and 252000 Da | Thermoanaerobacter italicus |
| 93000 | - |
1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE | Thermoanaerobacter italicus |
| 135000 | - |
1 * 135000, pectate lyase a, SDS-PAGE | Thermoanaerobacter italicus |
| 148000 | - |
pectate lyase a, non-denaturing PAGE | Thermoanaerobacter italicus |
| 158000 | - |
1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE | Thermoanaerobacter italicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter italicus | - |
nov., pectate lyase a and pectate lyase b | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pectate lyase a and pectate lyase b | Thermoanaerobacter italicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectate | - |
Thermoanaerobacter italicus | ? | - |
? | |
| pectin | - |
Thermoanaerobacter italicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE | Thermoanaerobacter italicus |
| monomer | 1 * 135000, pectate lyase a, SDS-PAGE | Thermoanaerobacter italicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
pectate lyase a | Thermoanaerobacter italicus |
| 80 | - |
pectate lyase b | Thermoanaerobacter italicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 85 | 60°C: about 55% of maximal activity, 80°C: about 90% of maximal activity, 85°C: about 20% of maximal activity, pectate lyase a | Thermoanaerobacter italicus |
| 60 | 90 | 60°C: about 45% of maximal activity, 90°C: about 30% of maximal activity, pectate lyase b | Thermoanaerobacter italicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
2 h, about 10% loss of activity | Thermoanaerobacter italicus |
| 80 | - |
half-life: about 60 min | Thermoanaerobacter italicus |
| 90 | - |
complete loss of activity after 5 min | Thermoanaerobacter italicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
pectate lyase a | Thermoanaerobacter italicus |
| 9 | - |
pectate lyase B | Thermoanaerobacter italicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity, pectate lyase a | Thermoanaerobacter italicus |
| 7 | 10 | pH 7.0: about 35% of maximal activity, pH 10.0: 50% of maximal activity, pectate lyase b | Thermoanaerobacter italicus |
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